Team:UT-Tokyo/Sudoku experiment

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Sudoku

Abstract Construct Lab note Result

Lab note

This is an abstract page. If you can see detail data, please click each protocols.

Pre-experiment

Pre-experiment

Make construct

Detail protocols about this is:

June

July

August

Check test

Check test


Number Name Link to BioBrick Plate coordinate Vector Code length
1 T7 promoter

[http://partsregistry.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074]

plate1-6N pSB1AK8 46bp
2 rbs

[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0030 BBa_B0030]

plate1-1H pSB1A2 15bp
3 cre recombinase

[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61047 BBa_J61047]

plate1-5D pSB1A2 1037bp
4 double terminator

[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0014 BBa_B0014]

plate2-24C pSB1AK3 95bp
5 lox66 recombinase site

[http://partsregistry.org/wiki/index.php?title=Part:BBa_I718017 BBa_I718017]

plate1-17J pSB1A2 34bp
6 Kan resistance (rev)

[http://partsregistry.org/wiki/index.php?title=Part:BBa_J31002 BBa_J31002]

plate1-2K pSB1A2 816bp
7 rbs (rev)

[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0014 BBa_B0014]

plate1-1J pSB1A2 15bp
8 lox71 recombinase site

order-made

--- --- 34bp
9 single terminator

[http://partsregistry.org/wiki/index.php?title=Part:BBa_B1006 BBa_B1006]

plate1-4H pSB1AK3 39bp
10 constant express promoter

[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 BBa_J23119]

plate1-18A pSB1A2 35bp
11 Tet resistance (rev)

[http://partsregistry.org/wiki/index.php?title=Part:BBa_J31006 BBa_J31006]

plate1-1N pSB1A2 1191bp
12 hixC

[http://partsregistry.org/wiki/index.php?title=Part:BBa_J44000 BBa_J44000]

plate1-1B pSB1A2 26bp
13 ---

---

--- --- ---
14 lox66

[http://partsregistry.org/wiki/index.php?title=Part:BBa_I718016 BBa_I718016]

plate1-17H pSB1A2 34bp
15 rbs-mRFP1-terminator

[http://partsregistry.org/wiki/index.php?title=Part:BBa_I13507 BBa_I13507]

plate1-22O pSB1A2 861bp
16 location sequence(old)

order

--- --- ---
17 pSP6(rev)

order

--- --- ---
18 lox2272

order

--- --- ---
19 loading sequence(rev)

order

--- --- ---
20 frt

[http://partsregistry.org/Part:BBa_J61020 BBa_J61020]

From HQ pSB1A2 34bp
21 hin(true)

[http://partsregistry.org/Part:BBa_J31000 BBa_J31000]

From HQ  ??? 573bp
22 hin(rev)

---

---  ??? 573bp
23 flpe

---

--- --- about 1.2kbp
24 gfp unit

---

--- --- about 1.2kbp
25 flpe(rev)

---

--- --- about 1.2kbp

Main construct

Sudoku's main construct

Research of SP6 RNApol cDNA and F plasmid

Research of SP6 RNApol cDNA and F plasmid

Convert lox71(rev) to lox66(rev)

Convert lox71(rev) to lox66(rev)

Make Hin(rev)

Make Hin(rev)

Revise MS2 gene

Revise MS2 gene

Make location(rev)-rbs(rev)-location(rev)

Make location(rev)-rbs(rev)-location(rev)


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