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Team:UT-Tokyo/Sudoku experiment
From 2010.igem.org
Sudoku
Abstract Construct Lab note Result
Lab note
This is an abstract page. If you can see detail data, please click each protocols.
Pre-experiment
Make construct
Detail protocols about this is:
Check test
Check test
| Number | Name | Link to BioBrick | Plate coordinate | Vector | Code length |
| 1 | T7 promoter |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074] | plate1-6N | pSB1AK8 | 46bp |
| 2 | rbs |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0030 BBa_B0030] | plate1-1H | pSB1A2 | 15bp |
| 3 | cre recombinase |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61047 BBa_J61047] | plate1-5D | pSB1A2 | 1037bp |
| 4 | double terminator |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0014 BBa_B0014] | plate2-24C | pSB1AK3 | 95bp |
| 5 | lox66 recombinase site |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I718017 BBa_I718017] | plate1-17J | pSB1A2 | 34bp |
| 6 | Kan resistance (rev) |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J31002 BBa_J31002] | plate1-2K | pSB1A2 | 816bp |
| 7 | rbs (rev) |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0014 BBa_B0014] | plate1-1J | pSB1A2 | 15bp |
| 8 | lox71 recombinase site |
order-made | --- | --- | 34bp |
| 9 | single terminator |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B1006 BBa_B1006] | plate1-4H | pSB1AK3 | 39bp |
| 10 | constant express promoter |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 BBa_J23119] | plate1-18A | pSB1A2 | 35bp |
| 11 | Tet resistance (rev) |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J31006 BBa_J31006] | plate1-1N | pSB1A2 | 1191bp |
| 12 | hixC |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J44000 BBa_J44000] | plate1-1B | pSB1A2 | 26bp |
| 13 | --- |
--- | --- | --- | --- |
| 14 | lox66 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I718016 BBa_I718016] | plate1-17H | pSB1A2 | 34bp |
| 15 | rbs-mRFP1-terminator |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I13507 BBa_I13507] | plate1-22O | pSB1A2 | 861bp |
| 16 | location sequence(old) |
order | --- | --- | --- |
| 17 | pSP6(rev) |
order | --- | --- | --- |
| 18 | lox2272 |
order | --- | --- | --- |
| 19 | loading sequence(rev) |
order | --- | --- | --- |
| 20 | frt |
[http://partsregistry.org/Part:BBa_J61020 BBa_J61020] | From HQ | pSB1A2 | 34bp |
| 21 | hin(true) |
[http://partsregistry.org/Part:BBa_J31000 BBa_J31000] | From HQ | ??? | 573bp |
| 22 | hin(rev) |
--- | --- | ??? | 573bp |
| 23 | flpe |
--- | --- | --- | about 1.2kbp |
| 24 | gfp unit |
--- | --- | --- | about 1.2kbp |
| 25 | flpe(rev) |
--- | --- | --- | about 1.2kbp |
Main construct
Research of SP6 RNApol cDNA and F plasmid
Research of SP6 RNApol cDNA and F plasmid
Convert lox71(rev) to lox66(rev)
Convert lox71(rev) to lox66(rev)
Make Hin(rev)
Make Hin(rev)
Revise MS2 gene
Revise MS2 gene
Make location(rev)-rbs(rev)-location(rev)
Make location(rev)-rbs(rev)-location(rev)
