Team:UT-Tokyo/Sudoku experiment
From 2010.igem.org
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=== Make construct === | === Make construct === | ||
- | Detail protocols about this is [https://2010.igem.org/Team:UT-Tokyo/Sudoku_notebook | + | Detail protocols about this is: [https://2010.igem.org/Team:UT-Tokyo/Sudoku_notebook July] |
- | + | [https://2010.igem.org/Team:UT-Tokyo/Sudoku_notebook_2 August]. | |
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- | + | ||
=== Check test === | === Check test === |
Revision as of 01:31, 19 September 2010
Sudoku
Abstract Construct Lab note Result
Lab note
This is an abstract page. If you can see detail data, please click each protocols. This is an abstract page. If you can see detail data, please click each protocols.
Pre-experiment
Make construct
Detail protocols about this is: July
Check test
Check test
Number | Name | Link to BioBrick | Plate coordinate | Vector | Code length |
1 | T7 promoter | plate1-6N | pSB1AK8 | 46bp | |
2 | rbs | plate1-1H | pSB1A2 | 15bp | |
3 | cre recombinase | plate1-5D | pSB1A2 | 1037bp | |
4 | double terminator | plate2-24C | pSB1AK3 | 95bp | |
5 | lox66 recombinase site | plate1-17J | pSB1A2 | 34bp | |
6 | Kan resistance (rev) | plate1-2K | pSB1A2 | 816bp | |
7 | rbs (rev) | plate1-1J | pSB1A2 | 15bp | |
8 | lox71 recombinase site |
order-made | --- | --- | 34bp |
9 | single terminator | plate1-4H | pSB1AK3 | 39bp | |
10 | constant express promoter | plate1-18A | pSB1A2 | 35bp | |
11 | Tet resistance (rev) | plate1-1N | pSB1A2 | 1191bp |
Main construct
Research of SP6 RNApol cDNA and F plasmid
Research of SP6 RNApol cDNA and F plasmid
Convert lox71(rev) to lox66(rev)
Convert lox71(rev) to lox66(rev)
Make Hin(rev)
Make Hin(rev)
Revise MS2 gene
Revise MS2 gene
Make location(rev)-rbs(rev)-location(rev)
Make location(rev)-rbs(rev)-location(rev)