Team:UT-Tokyo/Colony Pick Up
From 2010.igem.org
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+ | <h1>Colony Pick Up</h1> | ||
- | Preparation | + | <h2>Preparation</h2> |
*test tube | *test tube | ||
*LBbroth 4ml/tube | *LBbroth 4ml/tube | ||
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(take out from fridge and use after thawing out / needless to use after colony PCR) | (take out from fridge and use after thawing out / needless to use after colony PCR) | ||
- | Procedure | + | <h2>Procedure</h2> |
*1. add 2 μm of LB broth to the tube | *1. add 2 μm of LB broth to the tube | ||
*2. add the antibiotic to the tube(1μm for 1ml) | *2. add the antibiotic to the tube(1μm for 1ml) |
Latest revision as of 07:24, 23 September 2010
Colony Pick Up
Preparation
- test tube
- LBbroth 4ml/tube
- antibiotics
(take out from fridge and use after thawing out / needless to use after colony PCR)
Procedure
- 1. add 2 μm of LB broth to the tube
- 2. add the antibiotic to the tube(1μm for 1ml)
- 3. poke the colony on the plate by toothpick(or tip of pipette)and put the toothpick to the tube
- 4. label the cap of the tube and fix the cap on the tube
- 5. incubate it at 37 degrees (shake culture)
(15~18 hours is best / 8 hours is the fastest)
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