"
Team:Tsinghua/Notebook/4 September 2010
From 2010.igem.org
(Difference between revisions)
(New page: ==YX's part == PCR amplified the replication origin from pTKS/CS. Ligated to T vector as before. Transformed trans1-T1 and spread on Kanamycin LB plates) |
|||
| Line 5: | Line 5: | ||
Transformed trans1-T1 and spread on Kanamycin LB plates | Transformed trans1-T1 and spread on Kanamycin LB plates | ||
| + | |||
| + | == Module I, group 2c == | ||
| + | |||
| + | Pick several clones and culture them in 3ml LB with Amp respectively. | ||
| + | |||
| + | ---- | ||
| + | Extract plasmid from the clone we Picked yesterday. | ||
| + | Digest the plasmid with EcoRI and run gel to identify whether the clone is a positive one. | ||
| + | |||
| + | digestion system | ||
| + | plasmid 6ul | ||
| + | EcoRI 1ul | ||
| + | FD Buffer 2ul | ||
| + | H2O 11ul | ||
| + | |||
| + | Result: None of the clone picked is positive. | ||
Latest revision as of 21:12, 26 October 2010
YX's part
PCR amplified the replication origin from pTKS/CS.
Ligated to T vector as before.
Transformed trans1-T1 and spread on Kanamycin LB plates
Module I, group 2c
Pick several clones and culture them in 3ml LB with Amp respectively.
Extract plasmid from the clone we Picked yesterday. Digest the plasmid with EcoRI and run gel to identify whether the clone is a positive one.
digestion system
plasmid 6ul EcoRI 1ul FD Buffer 2ul H2O 11ul
Result: None of the clone picked is positive.
