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Team:Tsinghua/Notebook/1 September 2010
From 2010.igem.org
(Difference between revisions)
(New page: == YX's part == PCR amplified the second round of the resistance genes. Ligated to T vectors as before.) |
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| Line 3: | Line 3: | ||
Ligated to T vectors as before. | Ligated to T vectors as before. | ||
| + | |||
| + | == Module I, group 2c == | ||
| + | 12h after digestion, run a gel and cut the band conaining digested vector and purify it with kit. | ||
| + | ---- | ||
| + | Measure the concentration of the purified product. | ||
| + | ---- | ||
| + | Dephosphorylate the digested vector with AP. | ||
| + | ---- | ||
| + | system | ||
| + | AP 1ul | ||
| + | Buffer 2ul | ||
| + | plasmid 17ul | ||
| + | ---- | ||
| + | Incubate at 37°C for 2h and then purify the product with TIANGEN kit. | ||
Latest revision as of 21:01, 26 October 2010
YX's part
PCR amplified the second round of the resistance genes.
Ligated to T vectors as before.
Module I, group 2c
12h after digestion, run a gel and cut the band conaining digested vector and purify it with kit.
Measure the concentration of the purified product.
Dephosphorylate the digested vector with AP.
system
AP 1ul Buffer 2ul plasmid 17ul
Incubate at 37°C for 2h and then purify the product with TIANGEN kit.
