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From 2010.igem.org

Contents 1 1：DNA extraction 2 2：DNA Ligation 3 3:PCR 4 4:Transformation 5 5:Electrophoresis 6 6：DNA extraction 7 7：DNA Digestion 8 8:PCR

1：DNA extraction

＜Member＞
mizuki,Takahiro

＜Sample＞
PCR products

• 1(9/16)
• 2(9/16)
• 3(9/17)
• 4(9/16)
• 5(9/16)
• 7(9/16)

＜Protocol＞
See Protocol 4

2：DNA Ligation

＜Member＞
mio

＜Sample＞

• 7-8-10,vector
• 2,vector

(after digestion)

＜Protocol＞
See Protocol 3

3:PCR

<Member>
mizuki,Takahiro

<Sample>(temperature in annealing:℃)

• 1 (72)
• 2 (70)
• 4 (70)
• 5 (70)
• 9 (63,68)
• 10(58)
• vector(72)

<Protocol>
See Protocol 1

4:Transformation

<Member>
nito

<Sample>

• 7-8-10+vector
• 2+vector

<Protocol>
See Protocol 7

5:Electrophoresis

<Member>
nito,Takahiro

<Sample>
PCR products

• 1 (9/28)
• 2 (9/28)
• 4 (9/28)
• 5 (9/28)
• 8 (9/27)
• 9 (9/28)
• 10(9/28)
• vector

<Protocol>
See Protocol 8

<Result>
[[Image:]]

6：DNA extraction

＜Member＞
Takahiro

＜Sample＞
PCR products

＜Protocol＞
See Protocol 4

7：DNA Digestion

＜Member＞
Mariko

＜Sample, Materials＞
・PCR productions

• 1 (8/31)
• 2 (9/11)
• 4L(8/26)
• 5 (8/31)

・Digest enzyme

• Avr2
• Nhe1
• spe1

＜Protocol＞
See Prptpcpl 9

8:PCR

<Member>
Mariko

<Sample>(temperature in annealing:℃)

• 1 (72)
• 2 (70)
• 3 (69)
• 4 (70)

<Protocol>
See Protocol 1