Team:TU Delft/31 August 2010 content

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Emulsifier

Next week the plasmid with AlnA will arrive, so just to be sure I repeated the emulsifier assay which we designed to characterize the brick.

I have changed the previous setup slightly. To be brief:

  • Total volume 1 mL
  • 20 uL Sudan II in EtOH
  • Vortex 15 sec
  • Rest for 1 hour

The experiment was done 4 times.

TU Delft Emulsifier Assay Calibration.jpg

There obviously is a nice correlation between the concentration of SDS and the absorbance. Especially in het nanomolar region the distribution is almost perfectly lineair.

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