Team:TU Delft/21 July 2010 content
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Alkane degradation
Unfortunately there were no transformants on yesterday's plates. This is most likely due to the fact that the ligation didn't work with the small pieces of DNA of the RBSs. Next plan is to cut open the RBS plasmid without removing the RBS (with SpeI and PstI) and insert the gene in this plasmid. We will try this tomorrow.
Emulsifier
There were small colonies on the plates. Pieter picked seven and performed Colony PCR on them.Lane Description
# | Description | Expected lenght (bp) |
1 | EZ Ladder (5 μL) | |
2 | B0032 | 220 |
3 | Colony 1 | 300 |
4 | Colony 2 | 300 |
5 | Colony 3 | 300 |
6 | Colony 4 | 300 |
7 | Colony 5 | 300 |
8 | Colony 6 | 300 |
9 | Colony 7 | 300 |
In lane 2 we loaded the negative control (PCR Product of B0032). Unfortunately all other lanes were empty or the same as the negative control. So from this gel we conclude that the ligation has failed.