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From 2010.igem.org

Alkane degradation

For some reason, 009 and 010 just don't want to get into one plasmid. As a last resort we'll try every possible way to get the two together.

Digestions:

Lane #	BioBrick	Cut with	Extra	Buffer	BSA	Gel ok?
0	Smartladder (5μl)
D 1	009A	EcoRI, SpeI	AseI	Buffer 2 (NEB)	1x BSA	OK
D 2	009A	SpeI, PstI	Phosphatase	Buffer 2 (NEB)	1x BSA	OK
D 3	009A	SpeI, PstI	none	Buffer 2 (NEB)	1x BSA	OK
D 4	009A	EcoRI, XbaI	none	Buffer 2 (NEB)	1x BSA	OK
D 5	009A	XbaI, PstI	AseI	Buffer 2 (NEB)	1x BSA	OK
D 6	010A	EcoRI, SpeI	AseI	Buffer 2 (NEB)	1x BSA	OK
D 7	010A	SpeI, PstI	none	Buffer 2 (NEB)	1x BSA	OK
8	010A	EcoRI, XbaI	Phosphatase	Buffer 2 (NEB)	1x BSA	OK
D 9	010A	EcoRI, XbaI	none	Buffer 2 (NEB)	1x BSA	OK
D 10	010A	XbaI, PstI	AseI	Buffer 2 (NEB)	1x BSA	OK

Ligations:

'#	'Piece 1	Piece 2
028 L1	D1	D8
028 L2	D1	D9
028 L3	D2	D10
028 L4	D3	D10
028 L5	D4	D6
028 L6	D5	D7