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From 2010.igem.org

Meeting minutes

• Hugo - Maybe some issues with the measurements over the weekend – Thursday on Friday measurement went really well, those results can be uploaded. This was done with different concentrations of hexane
• Nadine – Salt tolerance, measured over the weekend (with LB and M9, concentrations of 0.6-0.9M NaCl)
• Hugo is going to do the characterization of alkane degradation; Friday, Ramon will help Hugo in the lab with GC measurements
• Do a digestion check of 017?
• Eva: 1C3 issues – grow and isolate again
• Luke: Pathway maps have been made coupled to MFA for on the wiki, but he should also explain the graphs in words (not only graphs)
  • It should be good to have a page that summarizes all the results, on which you can click to more information/details
• Jelmer: Software tool – almost done, results will be linked to prefoldin
• Nadine: Survey on the 25th, no plan yet for where, but questions already thought up, but Dirk Stemerding will

Alkane degradation

For some reason, 009 and 010 just don't want to get into one plasmid. As a last resort we'll try every possible way to get the two together.

Digestions:

Lane #	BioBrick	Cut with	Extra	Buffer	BSA	Gel ok?
0	Smartladder (5μl)
D 1	009A	EcoRI, SpeI	AseI	Buffer 2 (NEB)	1x BSA	OK
D 2	009A	SpeI, PstI	Phosphatase	Buffer 2 (NEB)	1x BSA	OK
D 3	009A	SpeI, PstI	none	Buffer 2 (NEB)	1x BSA	OK
D 4	009A	EcoRI, XbaI	none	Buffer 2 (NEB)	1x BSA	OK
D 5	009A	XbaI, PstI	AseI	Buffer 2 (NEB)	1x BSA	OK
D 6	010A	EcoRI, SpeI	AseI	Buffer 2 (NEB)	1x BSA	OK
D 7	010A	SpeI, PstI	none	Buffer 2 (NEB)	1x BSA	OK
8	010A	EcoRI, XbaI	Phosphatase	Buffer 2 (NEB)	1x BSA	OK
D 9	010A	EcoRI, XbaI	none	Buffer 2 (NEB)	1x BSA	OK
D 10	010A	XbaI, PstI	AseI	Buffer 2 (NEB)	1x BSA	OK

Ligations:

'#	'Piece 1	Piece 2
028 L1	D1	D8
028 L2	D1	D9
028 L3	D2	D10
028 L4	D3	D10
028 L5	D4	D6
028 L6	D5	D7