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Team:TU Delft/20 September 2010 content
From 2010.igem.org
(Difference between revisions)
(New page: ==Alkane degradation== For some reason, 009 and 010 just don't want to get into one plasmid. As a last resort we'll try every possible way to get the two together. Digestions: {| style=...) |
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{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | {| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | ||
|'''Lane #''' | |'''Lane #''' | ||
| - | |'''BioBrick'' | + | |'''BioBrick''' |
| - | |'''Cut with'' | + | |'''Cut with''' |
|'''Extra''' | |'''Extra''' | ||
|'''Buffer''' | |'''Buffer''' | ||
Revision as of 12:32, 1 October 2010
Alkane degradation
For some reason, 009 and 010 just don't want to get into one plasmid. As a last resort we'll try every possible way to get the two together.
Digestions:
| Lane # | BioBrick | Cut with | Extra | Buffer | BSA | Gel ok? |
| 0 | Smartladder (5μl) | |||||
| D 1 | 009A | EcoRI, SpeI | AseI | Buffer 2 (NEB) | 1x BSA | OK |
| D 2 | 009A | SpeI, PstI | Phosphatase | Buffer 2 (NEB) | 1x BSA | OK |
| D 3 | 009A | SpeI, PstI | none | Buffer 2 (NEB) | 1x BSA | OK |
| D 4 | 009A | EcoRI, XbaI | none | Buffer 2 (NEB) | 1x BSA | OK |
| D 5 | 009A | XbaI, PstI | AseI | Buffer 2 (NEB) | 1x BSA | OK |
| D 6 | 010A | EcoRI, SpeI | AseI | Buffer 2 (NEB) | 1x BSA | OK |
| D 7 | 010A | SpeI, PstI | none | Buffer 2 (NEB) | 1x BSA | OK |
| 8 | 010A | EcoRI, XbaI | Phosphatase | Buffer 2 (NEB) | 1x BSA | OK |
| D 9 | 010A | EcoRI, XbaI | none | Buffer 2 (NEB) | 1x BSA | OK |
| D 10 | 010A | XbaI, PstI | AseI | Buffer 2 (NEB) | 1x BSA | OK |
Ligations:
| '# | 'Piece 1 | Piece 2 |
| 028 L1 | D1 | D8 |
| 028 L2 | D1 | D9 |
| 028 L3 | D2 | D10 |
| 028 L4 | D3 | D10 |
| 028 L5 | D4 | D6 |
| 028 L6 | D5 | D7 |
