Team:SDU-Denmark/project-bc

From 2010.igem.org

(Difference between revisions)
(Model results)
(Model results)
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The time scale of the model might be to large, because it’s not possible to define units to the different parameters in the model such as defining time steps as seconds, minutes and so on, the problem involves defining concentrations of the enzymes.<br>
The time scale of the model might be to large, because it’s not possible to define units to the different parameters in the model such as defining time steps as seconds, minutes and so on, the problem involves defining concentrations of the enzymes.<br>
Some of the initial reaction rate constants and enzyme concentrations was from articles, and afterwards optimized for this model [[https://2010.igem.org/Team:SDU-Denmark/project-bc#References 3]].<br>
Some of the initial reaction rate constants and enzyme concentrations was from articles, and afterwards optimized for this model [[https://2010.igem.org/Team:SDU-Denmark/project-bc#References 3]].<br>
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[[Image: Team-SDU-Denmark-Madonnapic.pngTSRmodel.png |thumb|center|550px|'''Figure 5''': graph showing the concentration of phosphorylated CheB affecting the concentration of methylated TAR, making them drop.]] 
 
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Focusing on the behaviour of TAR/TARm in the model (Figure 5), it demonstrates that the concentration of methylated TAR rises if no phosphorylated CheB is present in the system.<br> When the concentration of phosphorylated CheB rises the concentration of methylated TAR drops.<br>
Focusing on the behaviour of TAR/TARm in the model (Figure 5), it demonstrates that the concentration of methylated TAR rises if no phosphorylated CheB is present in the system.<br> When the concentration of phosphorylated CheB rises the concentration of methylated TAR drops.<br>
This corresponded well with the known theory for the real system.
This corresponded well with the known theory for the real system.
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[[Image:Team-SDU-Denmark-CheAmodel.png |thumb|center|550px|'''Figure 6''': '''The top graph''' is showing the concentration of phosphorylated CheA rising when the concentration of methylated TSR drops. '''The bottom graph''' is showing the concentrations of CheB and CheY rising when the concentration of phosphorylated CheA rises.]]
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[[Image: Team-SDU-Denmark-Madonnapic.pngTSRmodel.png |thumb|center|550px|'''Figure 5''': graph showing the concentration of phosphorylated CheB affecting the concentration of methylated TAR, making them drop.]]  
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Focusing on the CheA/CheAp behaviour in the model (The top graph in figure 6), it demonstrates that the CheA auto-phophorylation rate rises when no TAR is methylated, thus producing phosphorylated CheA.<br>
Focusing on the CheA/CheAp behaviour in the model (The top graph in figure 6), it demonstrates that the CheA auto-phophorylation rate rises when no TAR is methylated, thus producing phosphorylated CheA.<br>
The dephosphorylation of CheA, is performed by both CheB and CheY (the bottom graph in figure 6), rise as the concentrations of both phosphorylated CheB and CheY rise and thereby decreases the concentration of phosphorylated CheA.<br>
The dephosphorylation of CheA, is performed by both CheB and CheY (the bottom graph in figure 6), rise as the concentrations of both phosphorylated CheB and CheY rise and thereby decreases the concentration of phosphorylated CheA.<br>
This corresponded well with the known theory for the real system.   
This corresponded well with the known theory for the real system.   
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[[Image:Team-SDU-Denmark-CheAmodel.png |thumb|center|550px|'''Figure 6''': '''The top graph''' is showing the concentration of phosphorylated CheA rising when the concentration of methylated TSR drops. '''The bottom graph''' is showing the concentrations of CheB and CheY rising when the concentration of phosphorylated CheA rises.]]
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Focusing on the CheB/CheBp behaviour in the model which illustrate that concentrations of phosphorylated CheB rises when phosphorylated CheA is present, as seen in figure 6 and its dephosphorylation occurs during the demethylation of TAR as seen in figure 5.<br>
Focusing on the CheB/CheBp behaviour in the model which illustrate that concentrations of phosphorylated CheB rises when phosphorylated CheA is present, as seen in figure 6 and its dephosphorylation occurs during the demethylation of TAR as seen in figure 5.<br>
This corresponded well with the known theory for the real system.<br><br>
This corresponded well with the known theory for the real system.<br><br>

Revision as of 23:07, 27 October 2010