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Team:SDU-Denmark/labnotes13
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== Flagella Group == | == Flagella Group == | ||
| + | |||
| + | == Retinal == | ||
| + | |||
| + | === PCR amplification of K343005 with dobbelt terminator === | ||
| + | Date: 8/10<br> | ||
| + | Done by: Marie<br> | ||
| + | Methods: PCR<br> | ||
| + | Protocols: CP1.1[https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1]<br> | ||
| + | Notes: <br> | ||
| + | 8 colonies were selected of colony 63 form colony PCR.<br> | ||
| + | VF2 and VR was used as primers in the PFU PCR<br> | ||
| + | PCR Program:<br> | ||
| + | <table style="text-align: left;" border="1" cellpadding="2" | ||
| + | cellspacing="2"> | ||
| + | <tr> | ||
| + | <td style="vertical-align: top;">Start<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">94 C<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">2 min<br> | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td style="vertical-align: top;">Denaturing<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">94 C<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">1 min<br> | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td style="vertical-align: top;">Annealing<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">55 C<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">1 min<br> | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td style="vertical-align: top;">Elongation<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">72 C<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">2:30 min<br> | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td style="vertical-align: top;">Goto2<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">rep<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">29x<br> | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td style="vertical-align: top;">End<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">72 C<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">5 min<br> | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td style="vertical-align: top;">Hold<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;">4 C<br> | ||
| + | </td> | ||
| + | <td style="vertical-align: top;"><br> | ||
| + | </td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | Afterwards a gel was run.<br> | ||
Latest revision as of 15:08, 16 October 2010
