Lab notes (9/27 - 10/3)

Flagella Group

Colony PCR of FlhDCmut and B0015 inserted into pSB3T5 with J13002

Date: 9/27 2010
Done By: Sheila
Protocol:
Notes: 34 colonies were tested. VF2 and VR primers were used.
Results:
Conclusion: Another round of colony PCR will be performed tomorrow. If this proves unsuccessful a new ligation and transformation will be performed.

Second colony PCR of FlhDCmut and B0015 inserted into pSB3T5 with J13002

Date: 9/28
Done By: Louise
Protocol:
Notes:
Results:
Conclusion:

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