Team:RMIT Australia/Modeling

From 2010.igem.org

(Difference between revisions)
(Taq DNA Polymerase)
(Taq DNA Polymerase)
Line 407: Line 407:
= Modelling =
= Modelling =
 +
 +
== Parameters used to compare the Models ==
 +
[[Image:Table.jpg]]
 +
== Taq DNA Polymerase ==
== Taq DNA Polymerase ==
 +
Taq Polymerase with no mutations
Taq Polymerase with no mutations
Line 445: Line 450:
The wild type Taq Polymerase was changed at the N and C terminal, with the Root Mean Square Deviation of 6.8834. This number indicates the measure of the average distance between the backbones of the two superimposed proteins.
The wild type Taq Polymerase was changed at the N and C terminal, with the Root Mean Square Deviation of 6.8834. This number indicates the measure of the average distance between the backbones of the two superimposed proteins.
-
 
-
The picture below shows the differences between the wild type Taq Polymerase and the redesigned Taq at the N and C terminus.
 
-
 
-
[[Image:Taq N.jpg|400px]]    [[Image:Taq C.jpg|400px]]
 
Our Taq Polymerase with mutations  
Our Taq Polymerase with mutations  
[[Image:Taq with labels.jpg]]
[[Image:Taq with labels.jpg]]
 +
 +
The picture below shows the differences between the wild type Taq Polymerase and the redesigned Taq at the N and C terminus.
 +
 +
[[Image:Taq N.jpg|400px]]    [[Image:Taq C.jpg|400px]]
== Fold-it==
== Fold-it==

Revision as of 08:21, 11 October 2010

Modelling

Parameters used to compare the Models

Table.jpg


Taq DNA Polymerase

Taq Polymerase with no mutations


WildTypetaq.jpgWildtype2.jpg


Aliphatic Index: 98.33

Ext Coefficient: 112760

Theoretical PI: 6.04

Instability Index: 32.93




We redesigned TAQ so it is suitable to undergo ligation independent cloning, to do this we changed the N and C terminal.

This is the redesigned Taq Polymerase

Taq part 1.jpg Taq part 2.jpg


Aliphatic Index: 96.46

Ext Coefficient: 107260

Theoretical PI: 6.09

Instability Index: 33.39


The wild type Taq Polymerase was changed at the N and C terminal, with the Root Mean Square Deviation of 6.8834. This number indicates the measure of the average distance between the backbones of the two superimposed proteins.

Our Taq Polymerase with mutations

Taq with labels.jpg

The picture below shows the differences between the wild type Taq Polymerase and the redesigned Taq at the N and C terminus.

Taq N.jpg Taq C.jpg

Fold-it

Fold-it.png


Foldit is a revolutionary new computer game enabling you to contribute to important scientific research. This page describes the science behind Foldit and how your playing can help. [http://fold.it/portal/ Click Here]