Latest revision as of 22:08, 27 October 2010

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iGEM Judging Comments

This year our team came up with a highly ambitious project and achieved goals in several different areas.

We successfully modelled, designed, characterised and submitted our IPTG-inducible Filamentous cells BioBrick part (BBa_K302012).

We also developed an e-Science Approach to Synthetic Biology which focuses on workflows in synthetic biology. This work led to the creation of BBF RFC 66.

We developed the Subtilin Immunity BioBrick, which provides immunity against the lantibiotic subtilin, a quorum sensing molecule for our cell population. Subtilin will help to initiate a population-wide response for concrete repair.

Our Urease BioBrick increases urea hydrolysis by increasing arginine and arginase production. Arginase breaks down arginine to form urea and ornithine. The overall increase in urea leads to an increase in urease production which hydrolyses urea into carbonate and ammonium ions which are exported out of the cell. The carbonate ions form a bond with calcium ions in the environment, resulting in the production of calcium carbonate.

Our Swarming BioBrick would help Bacillus subtilis 168 to swarm on the concrete surface by producing surfactin to reduce surface tension and by initiating flagellum biosynthesis.

Our Levan Glue BoBrick produces Levan glue in the presence of sucrose. The glue will act as a binding agent for the filamentous cells and the calcium carbonate crystals and will also help in filling up the crack thereby preventing corrosion of the steel reinforcements.

Our mazEF Kill switch is built around a stable toxin-antitoxin system for Bacillus subtilis. It would kill bacteria in the absence of sucrose thereby helping to make our project environmentally friendly.

In total we designed and entered 31 BioBrick parts in the parts registry.

We also took Scanning Electron Microscope photographs and found some interesting results: photographs of cells trying to fill up the crack, calcium carbonate crystals and Levan glue covering the cells.

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-iGEM 2010 will award medals.  All teams can earn models.  There are three levels of medals, from lowest to highest:
+='''iGEM Judging Comments'''=
-*Bronze Medal
-*Silver Medal
-*Gold Medal
-The requirements to earn a Bronze Medal are:
+This year our team came up with a highly ambitious project and achieved goals in several different areas.
-#Register the team, have a great summer, and have fun attending the Jamboree.
+We successfully modelled, designed, characterised and submitted our IPTG-inducible Filamentous cells BioBrick part ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K302012 BBa_K302012]).
-#Successfully complete and submit a Project Summary form.
-#Create and share a Description of the team's project via the iGEM wiki (see [https://2008.igem.org/Team:TUDelft TUDelft 2008] for a great example).
-#Present a Poster and Talk at the iGEM Jamboree (watch the [https://2008.igem.org/files/video/Heidelberg.mp4 Heidelberg 2008 video] for a great example).
-#Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Parts
-#*Entered information for each new part or device should at least include primary nucleic acid sequence, description of function, authorship, any relevant safety notes, and an acknowledgement of sources and references.  Consider [http://parts.mit.edu/registry/index.php/Part:BBa_J45004 BBa_J45004] as one example (be sure to check Main, Design Page, and Experiences sub-pages for this part).
-#*Teams are currently expected to design and contribute standard biological parts that conform to the accepted BioBrick standards for physical assembly. Non-BioBrick parts will not be recognized by iGEM 2010 judges unless they have [[Judging#BioBrick_standard_variance_requests | specific approval]].  The two specific BioBrick physical assembly schemes that the judges will recognize by default are (i) Tom Knight's [http://dspace.mit.edu/handle/1721.1/21168 original assembly standard] and (ii) Ira Phillips [http://dspace.mit.edu/handle/1721.1/32535 fusion assembly standard].
-#**[Special Note.  A discussion has been initiated by the BioBricks Standards Working Group to consider updating the BioBrick assembly standard in time for June 1.  Check back for any updates on acceptable BioBrick assembly standards.]
-#*Any new Devices that are based on gene expression are expected to conform to the PoPS device boundary standard.  See chapter 3 of the book, [http://openwetware.org/wiki/Adventures Adventures in Synthetic Biology], for more information about common signal carriers and PoPS.
-#Submit DNA for at least one new BioBrick Part or Device to the Registry of Parts.
-#*The submitted DNA must be associated with a Part or Device for which you have entered information describing the part or device, and must conform to the BioBrick standards for Parts or Devices (see above).
-The requirements to earn a Silver Medal, in addition to the Bronze Medal requirements, are:
+We also developed [https://2010.igem.org/Team:Newcastle/E-Science an e-Science Approach to Synthetic Biology] which focuses on workflows in synthetic biology. This work led to the creation of BBF RFC 66.
-#Demonstrate that at least one new BioBrick Part or Device of your own design and construction works as expected.
-#Characterize the operation of at least one new BioBrick Part or Device and enter this information on the Parts or Device page via the Registry of Parts (see [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] for an exemplar).
-The requirements to earn a Gold Medal, in addition to the Silver Medal requirements, are any one OR more of the following:
+We developed the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K302018 Subtilin Immunity] BioBrick, which provides immunity against the lantibiotic subtilin, a quorum sensing molecule for our cell population. Subtilin will help to initiate a population-wide response for concrete repair.
-#Characterize or improve an existing BioBrick Part or Device and enter this information back on the Registry.
-#Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.
+Our [http://partsregistry.org/wiki/index.php?title=Part:BBa_K302015 Urease] BioBrick increases urea hydrolysis by increasing arginine and arginase production. Arginase breaks down arginine to form urea and ornithine. The overall increase in urea leads to an increase in urease production which hydrolyses urea into carbonate and ammonium ions which are exported out of the cell. The carbonate ions form a bond with calcium ions in the environment, resulting in the production of calcium carbonate.
-#Develop and document a new technical standard that supports the (i) design of BioBrick Parts or Devices, or (ii) construction of BioBrick Parts or Devices, or (iii) characterization of BioBrick Parts or Devices, or (iv) analysis, modeling, and simulation of BioBrick Parts or Devices, or (v) sharing BioBrick Parts or Devices, either via physical DNA or as information via the internet.
-#Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.
+Our [http://partsregistry.org/wiki/index.php?title=Part:BBa_K302016 Swarming] BioBrick would help ''Bacillus subtilis'' 168 to swarm on the concrete surface by producing surfactin to reduce surface tension and by initiating flagellum biosynthesis.
+Our [http://partsregistry.org/wiki/index.php?title=Part:BBa_K302030 Levan Glue] BoBrick produces Levan glue in the presence of sucrose. The glue will act as a binding agent for the filamentous cells and the calcium carbonate crystals and will also help in filling up the crack thereby preventing corrosion of the steel reinforcements.
+Our [http://partsregistry.org/wiki/index.php?title=Part:BBa_K302035 ''mazEF'' Kill switch] is built around a stable toxin-antitoxin system for ''Bacillus subtilis''. It would kill bacteria in the absence of sucrose thereby helping to make our project environmentally friendly.
+In total we designed and entered 31 BioBrick parts in the parts registry.
+We also took [https://2010.igem.org/Team:Newcastle/solution#Scanning_Electron_Microscope_Images Scanning Electron Microscope photographs] and found some interesting results: photographs of cells trying to fill up the crack, calcium carbonate crystals and Levan glue covering the cells.
+{{Team:Newcastle/footer}}

Team:Newcastle/Medals

From 2010.igem.org

Latest revision as of 22:08, 27 October 2010

iGEM Judging Comments