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Team:Newcastle/IPTG INduction
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Shethharsh08 (Talk | contribs) (New page: =Aim= The aim of this protocol is to prepare slides for the ''Bacillus subtilis'' 168 cells which have ''yneA'' insertion and are induced by IPTG at various concentrations. =Materials= ...) |
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Revision as of 02:12, 26 October 2010
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Aim
The aim of this protocol is to prepare slides for the Bacillus subtilis 168 cells which have yneA insertion and are induced by IPTG at various concentrations.
Materials
- Flasks
- Colonies of Bacillus subtilis 168 cells with different plasmid insertions.
- Inoculation Loop
- Shaking incubator
- 1M IPTG stock solution
- Appropriate antibiotic solutions
- 1.2% agarose
- 12 well glass slide
   
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