Team:Newcastle/Gel electrophoresis


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Gel electrophoresis



  • Make up 1% agarose gel (1 g of agarose in 100 ml of TAE buffer) and transfer 60 ml of molten agarose gel into the gel tray with 3 μl of Safeview
  • Wait for 30 min to allow the gel to harden
  • Transfer harden gel into the gel tank and add TAE buffer until the gel is completely submerge
  • Depending on the nature of the sample, 3μl of GeneRuler™ 1kb Plus DNA Ladder was used for analysing DNA
  • Loading buffer was then added together with the sample before loading onto the gel matrix
  • Run gel at 90V until separation is achieved and visualize using the gelDoc