Team:Newcastle/Gel electrophoresis

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(New page: {{Team:Newcastle/mainbanner}} ===Gel electrophoresis=== *Transfer harden gel into the gel tank *add TAE buffer until the gel is completely submerged *Depending on the nature of the samp...)
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Revision as of 09:41, 27 July 2010

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Gel electrophoresis

  • Transfer harden gel into the gel tank
  • add TAE buffer until the gel is completely submerged
  • Depending on the nature of the sample, 3μl of GeneRuler™ 1kb Plus DNA Ladder
  • Loading buffer was then added together with the sample before loading onto the gel matrix
  • Run gel at 90V until separation is achieved and visualize using the gelDoc