Team:Newcastle/6 September 2010

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(New page: ===Aims=== rocF miniprep: we aim to purify rocF in p..... ===Materials and protocol=== See Mini prep protocol)
(Checking for transformants)
 
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===Aims===
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{{Team:Newcastle/mainbanner}}
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rocF miniprep: we aim to purify rocF in p.....
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=Checking for transformants=
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===Materials and protocol===
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Today we checked for transformants from the filamentous ''Bacillus subtilis'' transformation (with pMutin4) we performed yesterday.
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[[Team:Newcastle/Qiagen_Minipreps#Plasmid_extraction|See Mini prep protocol]]
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We checked our transformants under the light microscope, expecting to see a non-filamentous phenotype since the cells had not been induced with IPTG. The resutls were as expected.
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{{Team:Newcastle/footer}}

Latest revision as of 02:36, 28 October 2010

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Checking for transformants

Today we checked for transformants from the filamentous Bacillus subtilis transformation (with pMutin4) we performed yesterday.

We checked our transformants under the light microscope, expecting to see a non-filamentous phenotype since the cells had not been induced with IPTG. The resutls were as expected.

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