Team:Newcastle/6 September 2010

From 2010.igem.org

(Difference between revisions)
(Checking for transformants)
 
(32 intermediate revisions not shown)
Line 1: Line 1:
{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
-
=''roc''F=
 
-
===Aims===
 
-
''roc''F miniprep: we aim to purify ''roc''F in p.....
+
=Checking for transformants=
-
===Materials and protocol===
+
Today we checked for transformants from the filamentous ''Bacillus subtilis'' transformation (with pMutin4) we performed yesterday.
-
 
+
-
[[Team:Newcastle/Qiagen_Minipreps#Plasmid_extraction|See Mini prep protocol]]
+
-
 
+
-
===Results===
+
-
====Nanodrop====
+
-
''roc''F
+
-
 
+
-
Concentration of DNA in ng/µl
+
-
 
+
-
#93.1
+
-
#102.0
+
-
#99.4
+
-
#101.0
+
-
#98.8
+
-
#104.7
+
-
#93.1
+
-
#57.9
+
-
#81.9
+
-
 
+
-
 
+
-
 
+
-
=Subtilin Immunity BioBrick=
+
-
== Aims ==
+
-
Overnight cultures of all the colonies from the 'SI 1/9/10' plates was carried out today. 
+
-
 
+
-
== Methods ==
+
-
The [[Team:Newcastle/Growing_an_overnight_cultures| Growing Overnight Cultures]] protocol was followed. They were grown on LB with chloramphenicol. Altogether 16 colonies were produced on the plates and were left overnight. 
+
-
 
+
-
 
+
-
 
+
-
=Sucrose/Levan's glue=
+
-
The sucrose cultures and controls were compared. The cultures grown on sucrose should be considerably thicker.
+
-
The overnight cultures of the different strains were also grown on solid agar plates with and without sucrose.  
+
-
 
+
-
===PHOTOS===
+
 +
We checked our transformants under the light microscope, expecting to see a non-filamentous phenotype since the cells had not been induced with IPTG. The resutls were as expected.
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 02:36, 28 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Checking for transformants

Today we checked for transformants from the filamentous Bacillus subtilis transformation (with pMutin4) we performed yesterday.

We checked our transformants under the light microscope, expecting to see a non-filamentous phenotype since the cells had not been induced with IPTG. The resutls were as expected.

Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon