Latest revision as of 20:19, 25 October 2010

Research

We initiated our research on subtilin quorum sensing system which would provide a coordinated cell response.

Klein C, Kaletta C and Entian KD. (1993). "Biosynthesis of the lantibiotic subtilin is regulated by a histidine kinase/response regulator system". Applied and environmental microbiology. 59(1). 296-303.
Heinzmann S, Entian KD and Stein T. (2006). "Engineering Bacillus subtilis ATCC 6633 for improved production of the lantibiotic subtilin". Applied microbiology and biotechnology. 69(5). 532-6.

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-=LacI BioBrick Construction=
-==Aims==
+=Research=
-*To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
-==Materials==
+We initiated our research on subtilin quorum sensing system which would provide a coordinated cell response.
-*[[Team:Newcastle/14_July_2010|Second round of Transformants]]
-*[[Team:Newcastle/12_July_2010|Plasmid extracts]]
-*Restriction enzymes ''Xba''1 aand ''Pst''1
-==Protocol==
+#Klein C, Kaletta C and Entian KD. (1993). "Biosynthesis of the lantibiotic subtilin is regulated by a histidine kinase/response regulator system". ''Applied and environmental microbiology''. 59(1). 296-303.
-Single and Double digest of Plasmid extracts (single ''Xba''1)
+#Heinzmann S, Entian KD and Stein T. (2006). "Engineering Bacillus subtilis ATCC 6633 for improved production of the lantibiotic subtilin". ''Applied microbiology and biotechnology''. 69(5). 532-6.
-Run digests on a gel
-Overnight culture of second round of transformants
-==Results==
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