Subtilin Immunity BioBrick

Aims

Subtilin is required as a cell-signalling molecule in order to trigger calcium carbonate precipitation, as well as bacteria filament formation. We aim to produce 2 subtilin BioBricks; Production and Immunity. Because subtilin is a lantibiotic that is found naturally in Bacillus subtilis ATCC 6633, the introduction of subtilin into our Bacillus 168 cells would actually kill them. Therefore, it is essential to establish immunity for 168 strains.

Materials and Protocol

Please refer to the Phusion PCR protocol.