Team:Kyoto/Parts

From 2010.igem.org

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(K358000)
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The lac promoter will be repressed and not induce GFP in the presence of ''lac''I. We measured the strength of fluorescence with low copy plasmid vector, pSB4K5.
The lac promoter will be repressed and not induce GFP in the presence of ''lac''I. We measured the strength of fluorescence with low copy plasmid vector, pSB4K5.
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<partinfo>BBa_K358000 SpecifiedComponent</partinfo>
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<partinfo>BBa_K358000 SpecifiedComponents</partinfo>
====<partinfo>K358001</partinfo>====
====<partinfo>K358001</partinfo>====

Revision as of 11:53, 23 October 2010

Contents

Parts

Original

List

<groupparts>iGEM010 Kyoto</groupparts>

<partinfo>K358000</partinfo>

<partinfo>BBa_K358000 Short</partinfo>

The lac promoter will be repressed and not induce GFP in the presence of lacI. We measured the strength of fluorescence with low copy plasmid vector, pSB4K5.

<partinfo>BBa_K358000 SpecifiedComponents</partinfo>

<partinfo>K358001</partinfo>

<partinfo>BBa_K358001 short</partinfo>

constitutive promoter[J23101] with GFP on low plasmid[pSB4K5]

<partinfo>K358004</partinfo>

<partinfo>BBa_K358004 short</partinfo>

This part causes cell death. It contains holin/antiholin, endolysin and Rz/Rz1 genes.

<partinfo>K358006</partinfo>

<partinfo>BBa_K358006 short</partinfo>

λ lysis cassette <partinfo>BBa_K358004</partinfo> with double terminator <partinfo>BBa_B0015</partinfo>.

<partinfo>K358010</partinfo>

<partinfo>BBa_K358010 short</partinfo>

It codes SΔTMD1 as the anti-killer gene, which is derived from λ phage DNA and against the lambda lysis cassette[BBa_K358005]: the killer gene.

<partinfo>K358012</partinfo>

<partinfo>K358013</partinfo>

<partinfo>K358015</partinfo>

<partinfo>K358016</partinfo>

<partinfo>K358017</partinfo>

<partinfo>K358018</partinfo>

<partinfo>K358019</partinfo>

<partinfo>K358020</partinfo>

<partinfo>K358021</partinfo>

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BioBrick

Parts

NameDescriptionWell*1PlasmidResistanceInsert LengthVector Length
<partinfo>J23100</partinfo>Constitutive promoter family member1-18-C<partinfo>J61002</partinfo>Ampicillin352948
<partinfo>J23105</partinfo>Constitutive promoter family member1-18-M<partinfo>J61002</partinfo>Ampicillin352948
<partinfo>J23116</partinfo>Constitutive promoter family member1-20-M<partinfo>J61002</partinfo>Ampicillin352948
<partinfo>R0011</partinfo>Promoter (lacI regulated, lambda pL hybrid)1-6-G<partinfo>pSB1A2</partinfo>Ampicillin552079
<partinfo>B0015</partinfo>Double terminator (<partinfo>B0010</partinfo>-<partinfo>B0012</partinfo>)1-23-L<partinfo>pSB1AK3</partinfo>Ampicillin, Kanamycin1293189
<partinfo>E0840</partinfo>GFP generator1-12-O<partinfo>pSB1A2</partinfo>Ampicillin8782079
<partinfo>E0240</partinfo>GFP generator1-12-M<partinfo>pSB1A2</partinfo>Ampicillin8762079
<partinfo>I20260</partinfo>Measurement Kit Test of <partinfo>J23101</partinfo>2-17-F<partinfo>pSB3K3</partinfo>Kanamycin9192750
<partinfo>J06702</partinfo>mCherry, bacterial with RBS and forward terminator2-8-E<partinfo>pSB1A2</partinfo>Ampicillin8692079
<partinfo>pSB3K3</partinfo>RFP Coding Device1-5-E<partinfo>pSB3K3</partinfo>Kanamycin10692750
<partinfo>pSB4K5</partinfo>Low copy BioBrick standard vector1-5-G<partinfo>pSB4K5</partinfo>Kanamycin10693419
<partinfo>pSB1C3</partinfo>High copy BioBrick assembly plasmid<partinfo>pSB1C3</partinfo>Chloramphenicol2072
  • *1 "1-18-C" means well 18C in [http://partsregistry.org/Help:Spring_2010_DNA_distribution Spring 2010 DNA Distribution Kit] Plate 1.

Primers

NameDescriptionSequence
<partinfo>G00100</partinfo>Forward primer for sequencing/amplifying BioBrick parts (VF2)tgccacctgacgtctaagaa
<partinfo>G00101</partinfo>Reverse primer for sequencing/amplifying BioBrick parts (VR)attaccgcctttgagtgagc

^Top