Team:Kyoto/Parts

From 2010.igem.org

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(K358000)
(K358001)
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====<partinfo>K358001</partinfo>====
====<partinfo>K358001</partinfo>====
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<partinfo>BBa_K358001 short</partinfo>
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constitutive promoter[J23101] with GFP on low plasmid[pSB4K5]
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<partinfo>BBa_K358001 SequenceAndFeatures</partinfo>
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====<partinfo>K358004</partinfo>====
====<partinfo>K358004</partinfo>====
====<partinfo>K358006</partinfo>====
====<partinfo>K358006</partinfo>====

Revision as of 09:32, 23 October 2010

Contents

Parts

Original

List

<groupparts>iGEM010 Kyoto</groupparts>

<partinfo>K358000</partinfo>

<partinfo>BBa_K358000 short</partinfo>

The lac promoter will be repressed and not induce GFP in the presence of lacI. We measured the strength of fluorescence with low copy plasmid vector, pSB4K5.

<partinfo>BBa_K358000 SequenceAndFeatures</partinfo>

<partinfo>K358001</partinfo>

<partinfo>BBa_K358001 short</partinfo>

constitutive promoter[J23101] with GFP on low plasmid[pSB4K5]

<partinfo>BBa_K358001 SequenceAndFeatures</partinfo>

<partinfo>K358004</partinfo>

<partinfo>K358006</partinfo>

<partinfo>K358010</partinfo>

<partinfo>K358012</partinfo>

<partinfo>K358013</partinfo>

<partinfo>K358015</partinfo>

<partinfo>K358016</partinfo>

<partinfo>K358017</partinfo>

<partinfo>K358018</partinfo>

<partinfo>K358019</partinfo>

<partinfo>K358020</partinfo>

<partinfo>K358021</partinfo>

^Top

BioBrick

Parts

NameDescriptionWell*1PlasmidResistanceInsert LengthVector Length
<partinfo>J23100</partinfo>Constitutive promoter family member1-18-C<partinfo>J61002</partinfo>Ampicillin352948
<partinfo>J23105</partinfo>Constitutive promoter family member1-18-M<partinfo>J61002</partinfo>Ampicillin352948
<partinfo>J23116</partinfo>Constitutive promoter family member1-20-M<partinfo>J61002</partinfo>Ampicillin352948
<partinfo>R0011</partinfo>Promoter (lacI regulated, lambda pL hybrid)1-6-G<partinfo>pSB1A2</partinfo>Ampicillin552079
<partinfo>B0015</partinfo>Double terminator (<partinfo>B0010</partinfo>-<partinfo>B0012</partinfo>)1-23-L<partinfo>pSB1AK3</partinfo>Ampicillin, Kanamycin1293189
<partinfo>E0840</partinfo>GFP generator1-12-O<partinfo>pSB1A2</partinfo>Ampicillin8782079
<partinfo>E0240</partinfo>GFP generator1-12-M<partinfo>pSB1A2</partinfo>Ampicillin8762079
<partinfo>I20260</partinfo>Measurement Kit Test of <partinfo>J23101</partinfo>2-17-F<partinfo>pSB3K3</partinfo>Kanamycin9192750
<partinfo>J06702</partinfo>mCherry, bacterial with RBS and forward terminator2-8-E<partinfo>pSB1A2</partinfo>Ampicillin8692079
<partinfo>pSB3K3</partinfo>RFP Coding Device1-5-E<partinfo>pSB3K3</partinfo>Kanamycin10692750
<partinfo>pSB4K5</partinfo>Low copy BioBrick standard vector1-5-G<partinfo>pSB4K5</partinfo>Kanamycin10693419
<partinfo>pSB1C3</partinfo>High copy BioBrick assembly plasmid<partinfo>pSB1C3</partinfo>Chloramphenicol2072
  • *1 "1-18-C" means well 18C in [http://partsregistry.org/Help:Spring_2010_DNA_distribution Spring 2010 DNA Distribution Kit] Plate 1.

Primers

NameDescriptionSequence
<partinfo>G00100</partinfo>Forward primer for sequencing/amplifying BioBrick parts (VF2)tgccacctgacgtctaagaa
<partinfo>G00101</partinfo>Reverse primer for sequencing/amplifying BioBrick parts (VR)attaccgcctttgagtgagc

^Top