Team:Kyoto/Parts

From 2010.igem.org

(Difference between revisions)
(Parts)
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|<partinfo>J23116</partinfo>||Constitutive promoter family member||1-20-M||<partinfo>J61002</partinfo>||Ampicillin||35||2948
|<partinfo>J23116</partinfo>||Constitutive promoter family member||1-20-M||<partinfo>J61002</partinfo>||Ampicillin||35||2948
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|<partinfo>R0011</partinfo>||Promoter (''lacI'' regulated, lambda pL hybrid)||1-6-G||<partinfo>pSB1A2</partinfo>||Ampicillin||55||2079
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|<partinfo>R0011</partinfo>||Promoter (''lac''I regulated, lambda pL hybrid)||1-6-G||<partinfo>pSB1A2</partinfo>||Ampicillin||55||2079
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|<partinfo>B0015</partinfo>||Double terminator (<partinfo>B0010</partinfo>-<partinfo>B0012</partinfo>)||1-23-L||<partinfo>pSB1AK3</partinfo>||Ampicillin, Kanamycin||129||3189
|<partinfo>B0015</partinfo>||Double terminator (<partinfo>B0010</partinfo>-<partinfo>B0012</partinfo>)||1-23-L||<partinfo>pSB1AK3</partinfo>||Ampicillin, Kanamycin||129||3189

Revision as of 06:26, 21 October 2010

Contents

Parts

Original

<groupparts>iGEM010 Kyoto</groupparts>

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BioBrick

Parts

NameDescriptionWell*1PlasmidResistanceInsert LengthVector Length
<partinfo>J23100</partinfo>Constitutive promoter family member1-18-C<partinfo>J61002</partinfo>Ampicillin352948
<partinfo>J23105</partinfo>Constitutive promoter family member1-18-M<partinfo>J61002</partinfo>Ampicillin352948
<partinfo>J23116</partinfo>Constitutive promoter family member1-20-M<partinfo>J61002</partinfo>Ampicillin352948
<partinfo>R0011</partinfo>Promoter (lacI regulated, lambda pL hybrid)1-6-G<partinfo>pSB1A2</partinfo>Ampicillin552079
<partinfo>B0015</partinfo>Double terminator (<partinfo>B0010</partinfo>-<partinfo>B0012</partinfo>)1-23-L<partinfo>pSB1AK3</partinfo>Ampicillin, Kanamycin1293189
<partinfo>E0840</partinfo>GFP generator1-12-O<partinfo>pSB1A2</partinfo>Ampicillin8782079
<partinfo>E0240</partinfo>GFP generator1-12-M<partinfo>pSB1A2</partinfo>Ampicillin8762079
<partinfo>I20260</partinfo>Measurement Kit Test of <partinfo>J23101</partinfo>2-17-F<partinfo>pSB3K3</partinfo>Kanamycin9192750
<partinfo>J06702</partinfo>mCherry, bacterial with RBS and forward terminator2-8-E<partinfo>pSB1A2</partinfo>Ampicillin8692079
<partinfo>pSB3K3</partinfo>RFP Coding Device1-5-E<partinfo>pSB3K3</partinfo>Kanamycin10692750
<partinfo>pSB4K5</partinfo>Low copy BioBrick standard vector1-5-G<partinfo>pSB4K5</partinfo>Kanamycin10693419
<partinfo>pSB1C3</partinfo>High copy BioBrick assembly plasmid<partinfo>pSB1C3</partinfo>Chloramphenicol2072
  • *1 "1-18-C" means well 18C in [http://partsregistry.org/Help:Spring_2010_DNA_distribution Spring 2010 DNA Distribution Kit] Plate 1.

Primers

NameDescriptionSequence
<partinfo>G00100</partinfo>Forward primer for sequencing/amplifying BioBrick parts (VF2)tgccacctgacgtctaagaa
<partinfo>G00101</partinfo>Reverse primer for sequencing/amplifying BioBrick parts (VR)attaccgcctttgagtgagc

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