Team:Kyoto/Materials

From 2010.igem.org

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{| class="materials"
{| class="materials"
!Name||Genotype and/or Relevant Features||Source or Reference
!Name||Genotype and/or Relevant Features||Source or Reference
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|C2||||
 
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|TOP10||F-, ''mcr''A, &Delta;(''mrr''-''hsd''RMS-''mcr''BC), &phi;80''lac''Z&Delta;M15, &Delta;''lac''X74, ''nup''G, ''rec''A1, ''ara''D139, &Delta;(''ara''-''leu'')7697, ''gal''E15, ''gal''K16 ''rps''L(Str<sup>R</sup>), ''end''A1, &lambda;-||Invitrogen
|TOP10||F-, ''mcr''A, &Delta;(''mrr''-''hsd''RMS-''mcr''BC), &phi;80''lac''Z&Delta;M15, &Delta;''lac''X74, ''nup''G, ''rec''A1, ''ara''D139, &Delta;(''ara''-''leu'')7697, ''gal''E15, ''gal''K16 ''rps''L(Str<sup>R</sup>), ''end''A1, &lambda;-||Invitrogen
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|KRX||[F&prime;, ''tra''D36, &Delta;''omp''P, ''pro''A<sup>+</sup>B<sup>+</sup>, ''lac''I<sup>q</sup>, &Delta;(''lac''Z)M15] &Delta;''omp''T, ''end''A1, ''rec''A1, ''gyr''A96 (Nal<sup>r</sup>), ''thi''-1, ''hsd''R17 (r<sub>k</sub><sup>–</sup>, m<sub>k</sub><sup>+</sup>), e14– (McrA–), ''rel''A1, ''sup''E44, &Delta;(''lac''-''pro''AB), &Delta;(''rha''BAD)::T7 RNA polymerase.||Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1698]
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|rowspan="2"|KRX||rowspan="2"|[F&prime;, ''tra''D36, &Delta;''omp''P, ''pro''A<sup>+</sup>B<sup>+</sup>, ''lac''I<sup>q</sup>, &Delta;(''lac''Z)M15] &Delta;''omp''T, ''end''A1, ''rec''A1, ''gyr''A96 (Nal<sup>r</sup>), ''thi''-1, ''hsd''R17 (r<sub>k</sub><sup>–</sup>, m<sub>k</sub><sup>+</sup>), e14– (McrA–), ''rel''A1, ''sup''E44, &Delta;(''lac''-''pro''AB), &Delta;(''rha''BAD)::T7 RNA polymerase.||Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University
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|(Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1698])
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|BL21(DE3)pLysS||F–, ''omp''T, ''hsd''S<sub>B</sub> (r<sub>B</sub><sup>–</sup>, m<sub>B</sub><sup>–</sup>), ''dcm'', ''gal'', &lambda;(DE3), pLysS, Cm<sup>r</sup>.||Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1005]
|BL21(DE3)pLysS||F–, ''omp''T, ''hsd''S<sub>B</sub> (r<sub>B</sub><sup>–</sup>, m<sub>B</sub><sup>–</sup>), ''dcm'', ''gal'', &lambda;(DE3), pLysS, Cm<sup>r</sup>.||Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1005]
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!Name||Source or Reference
!Name||Source or Reference
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|Lambda Phage (cI857 Sam7)||DNA Digested Markers (#3403 &lambda;-Hind III digest) from Takara Bio [http://catalog.takara-bio.co.jp/en/product/basic_info.asp?unitid=U100005399]
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|rowspan="2"|Lambda Phage (cI857 Sam7)||DNA Digested Markers (#3403 &lambda;-Hind III digest) from Takara Bio [http://catalog.takara-bio.co.jp/en/product/basic_info.asp?unitid=U100005399]
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|http://www.ncbi.nlm.nih.gov/nuccore/NC_001416
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|08/20||SRRz Reverse Retry||CTGCAGCGGCCGCTACTAGTAgcaactctatctgcactgctcattaatatacttctgg
|08/20||SRRz Reverse Retry||CTGCAGCGGCCGCTACTAGTAgcaactctatctgcactgctcattaatatacttctgg
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|08/23, 08/24||Deletion for Upstream Forward||gagcaaatccccttattgggggtaagacatg
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|08/23, 08/24||Deletion of Upstream Forward||gagcaaatccccttattgggggtaagacatg
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|08/23, 08/24||Deletion for Upstream Reverse||ctctagaagcggccgcgaattcc
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|08/23, 08/24||Deletion of Upstream Reverse||ctctagaagcggccgcgaattcc
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|09/13, 09/22||Add J23105 Forward||CAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc
|09/13, 09/22||Add J23105 Forward||CAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc
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|09/13, 09/22||Add J23105 Reverse||AGCTAGCCGTAAActctagtatataaacgcagaaaggcccacc
|09/13, 09/22||Add J23105 Reverse||AGCTAGCCGTAAActctagtatataaacgcagaaaggcccacc
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|-
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| - ||VF2 (<partinfo>G00100</partinfo>)||tgccacctgacgtctaagaa
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|10/10||Deletion of GFP Forward (Primer1)||ccaggcatcaaataaaacgaaaggctc
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| - ||VF (<partinfo>G00101</partinfo>)||attaccgcctttgagtgagc
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|10/10||Deletion of GFP-DT Forward (Primer2)||tactagtagcggccgctgc
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===Reagent===
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{| class="materials"
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!Name||Supplier
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|Ampicillin||
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|10/10||Deletion of GFP, GFP-DT Reverse (Primer3)||ctctagtattattgatttctaccatcttctactcc
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|Kanamycin||
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| - ||VF2 (<partinfo>G00100</partinfo>)||tgccacctgacgtctaagaa
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|Chloramphenicol||
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| - ||VF (<partinfo>G00101</partinfo>)||attaccgcctttgagtgagc
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|EcoRI||
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|XbaI||
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|SpeI||
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|PstI||
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|DpnI||
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|Isopropyl &beta;-D-1-Thiogalactopyranoside (IPTG)||
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|Ligation High||
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|}
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Latest revision as of 04:24, 26 October 2010

Contents

Materials

Strains

NameGenotype and/or Relevant FeaturesSource or Reference
TOP10F-, mcrA, Δ(mrr-hsdRMS-mcrBC), φ80lacZΔM15, ΔlacX74, nupG, recA1, araD139, Δ(ara-leu)7697, galE15, galK16 rpsL(StrR), endA1, λ-Invitrogen
KRX[F′, traD36, ΔompP, proA+B+, lacIq, Δ(lacZ)M15] ΔompT, endA1, recA1, gyrA96 (Nalr), thi-1, hsdR17 (rk–, mk+), e14– (McrA–), relA1, supE44, Δ(lac-proAB), Δ(rhaBAD)::T7 RNA polymerase.Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University
(Promega [1])
BL21(DE3)pLysSF–, ompT, hsdSB (rB–, mB–), dcm, gal, λ(DE3), pLysS, Cmr.Promega [2]

DNA

NameSource or Reference
Lambda Phage (cI857 Sam7)DNA Digested Markers (#3403 λ-Hind III digest) from Takara Bio [3]
http://www.ncbi.nlm.nih.gov/nuccore/NC_001416

Primers

DateNameSequence
07/21S Forward (Same as SRRz Forward)gGAATTCGCGGCCGCTTCTAGAGgcctggatttgttctatcagtaatcga
07/21S ReverseCTGCAGCGGCCGCTACTAGTAttattgatttctaccatcttctactccggc
07/21SRRz ReverseCTGCAGCGGCCGCTACTAGTAtgagttgcccatcgatatgggc
07/28ΔTMD1 Forwardcgcggcagatataatggcggt
07/28ΔTMD1 Reversegtcatgtttttctggcatcttcatgtct
08/19, 08/24Sam7 Forwardggttcattcgtgaccttctcgacttc
08/19, 08/24Sam7 Reverseaggcgataatggcgcacatc
08/20SRRz Forward RetrygGAATTCGCGGCCGCTTCTAGAGgagcaaatccccttattgggggtaagacatg
08/20SRRz Reverse RetryCTGCAGCGGCCGCTACTAGTAgcaactctatctgcactgctcattaatatacttctgg
08/23, 08/24Deletion of Upstream Forwardgagcaaatccccttattgggggtaagacatg
08/23, 08/24Deletion of Upstream Reversectctagaagcggccgcgaattcc
09/13, 09/22Add J23105 ForwardCAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc
09/13, 09/22Add J23105 ReverseAGCTAGCCGTAAActctagtatataaacgcagaaaggcccacc
10/10Deletion of GFP Forward (Primer1)ccaggcatcaaataaaacgaaaggctc
10/10Deletion of GFP-DT Forward (Primer2)tactagtagcggccgctgc
10/10Deletion of GFP, GFP-DT Reverse (Primer3)ctctagtattattgatttctaccatcttctactcc
- VF2 (<partinfo>G00100</partinfo>)tgccacctgacgtctaagaa
- VF (<partinfo>G00101</partinfo>)attaccgcctttgagtgagc
Retrieved from "http://2010.igem.org/Team:Kyoto/Materials"