Team:Kyoto/Materials
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{{:Team:Kyoto/Header}} | {{:Team:Kyoto/Header}} | ||
==Materials== | ==Materials== | ||
- | ==Strains== | + | ===Strains=== |
{| class="materials" | {| class="materials" | ||
!Name||Genotype and/or Relevant Features||Source or Reference | !Name||Genotype and/or Relevant Features||Source or Reference | ||
- | |||
- | |||
|- | |- | ||
|TOP10||F-, ''mcr''A, Δ(''mrr''-''hsd''RMS-''mcr''BC), φ80''lac''ZΔM15, Δ''lac''X74, ''nup''G, ''rec''A1, ''ara''D139, Δ(''ara''-''leu'')7697, ''gal''E15, ''gal''K16 ''rps''L(Str<sup>R</sup>), ''end''A1, λ-||Invitrogen | |TOP10||F-, ''mcr''A, Δ(''mrr''-''hsd''RMS-''mcr''BC), φ80''lac''ZΔM15, Δ''lac''X74, ''nup''G, ''rec''A1, ''ara''D139, Δ(''ara''-''leu'')7697, ''gal''E15, ''gal''K16 ''rps''L(Str<sup>R</sup>), ''end''A1, λ-||Invitrogen | ||
|- | |- | ||
- | |KRX||[F′, ''tra''D36, Δ''omp''P, ''pro''A<sup>+</sup>B<sup>+</sup>, ''lac''I<sup>q</sup>, Δ(''lac''Z)M15] Δ''omp''T, ''end''A1, ''rec''A1, ''gyr''A96 (Nal<sup>r</sup>), ''thi''-1, ''hsd''R17 (r<sub>k</sub><sup>–</sup>, m<sub>k</sub><sup>+</sup>), e14– (McrA–), ''rel''A1, ''sup''E44, Δ(''lac''-''pro''AB), Δ(''rha''BAD)::T7 RNA polymerase.||Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1698] | + | |rowspan="2"|KRX||rowspan="2"|[F′, ''tra''D36, Δ''omp''P, ''pro''A<sup>+</sup>B<sup>+</sup>, ''lac''I<sup>q</sup>, Δ(''lac''Z)M15] Δ''omp''T, ''end''A1, ''rec''A1, ''gyr''A96 (Nal<sup>r</sup>), ''thi''-1, ''hsd''R17 (r<sub>k</sub><sup>–</sup>, m<sub>k</sub><sup>+</sup>), e14– (McrA–), ''rel''A1, ''sup''E44, Δ(''lac''-''pro''AB), Δ(''rha''BAD)::T7 RNA polymerase.||Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University |
+ | |- | ||
+ | |(Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1698]) | ||
|- | |- | ||
|BL21(DE3)pLysS||F–, ''omp''T, ''hsd''S<sub>B</sub> (r<sub>B</sub><sup>–</sup>, m<sub>B</sub><sup>–</sup>), ''dcm'', ''gal'', λ(DE3), pLysS, Cm<sup>r</sup>.||Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1005] | |BL21(DE3)pLysS||F–, ''omp''T, ''hsd''S<sub>B</sub> (r<sub>B</sub><sup>–</sup>, m<sub>B</sub><sup>–</sup>), ''dcm'', ''gal'', λ(DE3), pLysS, Cm<sup>r</sup>.||Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1005] | ||
|} | |} | ||
- | === | + | ===DNA=== |
{| class="materials" | {| class="materials" | ||
- | ! | + | !Name||Source or Reference |
|- | |- | ||
- | |rowspan=" | + | |rowspan="2"|Lambda Phage (cI857 Sam7)||DNA Digested Markers (#3403 λ-Hind III digest) from Takara Bio [http://catalog.takara-bio.co.jp/en/product/basic_info.asp?unitid=U100005399] |
|- | |- | ||
- | | | + | |http://www.ncbi.nlm.nih.gov/nuccore/NC_001416 |
+ | |} | ||
+ | |||
+ | ===Primers=== | ||
+ | {| class="materials" | ||
+ | !Date||Name||Sequence | ||
|- | |- | ||
- | | | + | |07/21||S Forward (Same as SRRz Forward)||gGAATTCGCGGCCGCTTCTAGAGgcctggatttgttctatcagtaatcga |
|- | |- | ||
- | | | + | |07/21||S Reverse||CTGCAGCGGCCGCTACTAGTAttattgatttctaccatcttctactccggc |
|- | |- | ||
- | | | + | |07/21||SRRz Reverse||CTGCAGCGGCCGCTACTAGTAtgagttgcccatcgatatgggc |
|- | |- | ||
- | | | + | |07/28||ΔTMD1 Forward||cgcggcagatataatggcggt |
|- | |- | ||
- | | | + | |07/28||ΔTMD1 Reverse||gtcatgtttttctggcatcttcatgtct |
|- | |- | ||
- | | | + | |08/19, 08/24||Sam7 Forward||ggttcattcgtgaccttctcgacttc |
|- | |- | ||
- | | | + | |08/19, 08/24||Sam7 Reverse||aggcgataatggcgcacatc |
|- | |- | ||
- | | | + | |08/20||SRRz Forward Retry||gGAATTCGCGGCCGCTTCTAGAGgagcaaatccccttattgggggtaagacatg |
+ | |- | ||
+ | |08/20||SRRz Reverse Retry||CTGCAGCGGCCGCTACTAGTAgcaactctatctgcactgctcattaatatacttctgg | ||
+ | |- | ||
+ | |08/23, 08/24||Deletion of Upstream Forward||gagcaaatccccttattgggggtaagacatg | ||
+ | |- | ||
+ | |08/23, 08/24||Deletion of Upstream Reverse||ctctagaagcggccgcgaattcc | ||
+ | |- | ||
+ | |09/13, 09/22||Add J23105 Forward||CAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc | ||
+ | |- | ||
+ | |09/13, 09/22||Add J23105 Reverse||AGCTAGCCGTAAActctagtatataaacgcagaaaggcccacc | ||
+ | |- | ||
+ | |10/10||Deletion of GFP Forward (Primer1)||ccaggcatcaaataaaacgaaaggctc | ||
+ | |- | ||
+ | |10/10||Deletion of GFP-DT Forward (Primer2)||tactagtagcggccgctgc | ||
+ | |- | ||
+ | |10/10||Deletion of GFP, GFP-DT Reverse (Primer3)||ctctagtattattgatttctaccatcttctactcc | ||
+ | |- | ||
+ | | - ||VF2 (<partinfo>G00100</partinfo>)||tgccacctgacgtctaagaa | ||
+ | |- | ||
+ | | - ||VF (<partinfo>G00101</partinfo>)||attaccgcctttgagtgagc | ||
|} | |} | ||
- | |||
---- | ---- |
Latest revision as of 04:24, 26 October 2010
Contents |
Materials
Strains
Name | Genotype and/or Relevant Features | Source or Reference |
---|---|---|
TOP10 | F-, mcrA, Δ(mrr-hsdRMS-mcrBC), φ80lacZΔM15, ΔlacX74, nupG, recA1, araD139, Δ(ara-leu)7697, galE15, galK16 rpsL(StrR), endA1, λ- | Invitrogen |
KRX | [F′, traD36, ΔompP, proA+B+, lacIq, Δ(lacZ)M15] ΔompT, endA1, recA1, gyrA96 (Nalr), thi-1, hsdR17 (rk–, mk+), e14– (McrA–), relA1, supE44, Δ(lac-proAB), Δ(rhaBAD)::T7 RNA polymerase. | Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University |
(Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1698]) | ||
BL21(DE3)pLysS | F–, ompT, hsdSB (rB–, mB–), dcm, gal, λ(DE3), pLysS, Cmr. | Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1005] |
DNA
Name | Source or Reference |
---|---|
Lambda Phage (cI857 Sam7) | DNA Digested Markers (#3403 λ-Hind III digest) from Takara Bio [http://catalog.takara-bio.co.jp/en/product/basic_info.asp?unitid=U100005399] |
http://www.ncbi.nlm.nih.gov/nuccore/NC_001416 |
Primers
Date | Name | Sequence |
---|---|---|
07/21 | S Forward (Same as SRRz Forward) | gGAATTCGCGGCCGCTTCTAGAGgcctggatttgttctatcagtaatcga |
07/21 | S Reverse | CTGCAGCGGCCGCTACTAGTAttattgatttctaccatcttctactccggc |
07/21 | SRRz Reverse | CTGCAGCGGCCGCTACTAGTAtgagttgcccatcgatatgggc |
07/28 | ΔTMD1 Forward | cgcggcagatataatggcggt |
07/28 | ΔTMD1 Reverse | gtcatgtttttctggcatcttcatgtct |
08/19, 08/24 | Sam7 Forward | ggttcattcgtgaccttctcgacttc |
08/19, 08/24 | Sam7 Reverse | aggcgataatggcgcacatc |
08/20 | SRRz Forward Retry | gGAATTCGCGGCCGCTTCTAGAGgagcaaatccccttattgggggtaagacatg |
08/20 | SRRz Reverse Retry | CTGCAGCGGCCGCTACTAGTAgcaactctatctgcactgctcattaatatacttctgg |
08/23, 08/24 | Deletion of Upstream Forward | gagcaaatccccttattgggggtaagacatg |
08/23, 08/24 | Deletion of Upstream Reverse | ctctagaagcggccgcgaattcc |
09/13, 09/22 | Add J23105 Forward | CAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc |
09/13, 09/22 | Add J23105 Reverse | AGCTAGCCGTAAActctagtatataaacgcagaaaggcccacc |
10/10 | Deletion of GFP Forward (Primer1) | ccaggcatcaaataaaacgaaaggctc |
10/10 | Deletion of GFP-DT Forward (Primer2) | tactagtagcggccgctgc |
10/10 | Deletion of GFP, GFP-DT Reverse (Primer3) | ctctagtattattgatttctaccatcttctactcc |
- | VF2 (<partinfo>G00100</partinfo>) | tgccacctgacgtctaagaa |
- | VF (<partinfo>G00101</partinfo>) | attaccgcctttgagtgagc |