"
Team:Kyoto/Materials
From 2010.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
{{:Team:Kyoto/Header}} | {{:Team:Kyoto/Header}} | ||
| - | =Materials= | + | ==Materials== |
| - | + | ==Strains== | |
{| class="materials" | {| class="materials" | ||
!Name||Genotype and/or Relevant Features||Source or Reference | !Name||Genotype and/or Relevant Features||Source or Reference | ||
| Line 14: | Line 14: | ||
|} | |} | ||
| - | + | ==DNA== | |
{| class="materials" | {| class="materials" | ||
!Name||Source or Reference | !Name||Source or Reference | ||
| Line 21: | Line 21: | ||
|} | |} | ||
| - | + | ==Primers== | |
{| class="materials" | {| class="materials" | ||
!Date||Name||Sequence | !Date||Name||Sequence | ||
| Line 56: | Line 56: | ||
|} | |} | ||
| - | + | ==Reagent== | |
{| class="materials" | {| class="materials" | ||
!Name||Supplier | !Name||Supplier | ||
Revision as of 18:39, 20 October 2010
LastModified: 2010-10-20
Contents |
Materials
Strains
| Name | Genotype and/or Relevant Features | Source or Reference |
|---|---|---|
| C2 | ||
| TOP10 | F-, mcrA, Δ(mrr-hsdRMS-mcrBC), φ80lacZΔM15, ΔlacX74, nupG, recA1, araD139, Δ(ara-leu)7697, galE15, galK16 rpsL(StrR), endA1, λ- | Invitrogen |
| KRX | [F′, traD36, ΔompP, proA+B+, lacIq, Δ(lacZ)M15] ΔompT, endA1, recA1, gyrA96 (Nalr), thi-1, hsdR17 (rk–, mk+), e14– (McrA–), relA1, supE44, Δ(lac-proAB), Δ(rhaBAD)::T7 RNA polymerase. | Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1698] |
| BL21(DE3)pLysS | F–, ompT, hsdSB (rB–, mB–), dcm, gal, λ(DE3), pLysS, Cmr. | Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1005] |
DNA
| Name | Source or Reference |
|---|---|
| Lambda Phage (cI857 Sam7) | DNA Digested Markers (#3403 λ-Hind III digest) from Takara Bio [http://catalog.takara-bio.co.jp/en/product/basic_info.asp?unitid=U100005399] |
Primers
| Date | Name | Sequence |
|---|---|---|
| 07/21 | S Forward (Same as SRRz Forward) | gGAATTCGCGGCCGCTTCTAGAGgcctggatttgttctatcagtaatcga |
| 07/21 | S Reverse | CTGCAGCGGCCGCTACTAGTAttattgatttctaccatcttctactccggc |
| 07/21 | SRRz Reverse | CTGCAGCGGCCGCTACTAGTAtgagttgcccatcgatatgggc |
| 07/28 | ΔTMD1 Forward | cgcggcagatataatggcggt |
| 07/28 | ΔTMD1 Reverse | gtcatgtttttctggcatcttcatgtct |
| 08/19, 08/24 | Sam7 Forward | ggttcattcgtgaccttctcgacttc |
| 08/19, 08/24 | Sam7 Reverse | aggcgataatggcgcacatc |
| 08/20 | SRRz Forward Retry | gGAATTCGCGGCCGCTTCTAGAGgagcaaatccccttattgggggtaagacatg |
| 08/20 | SRRz Reverse Retry | CTGCAGCGGCCGCTACTAGTAgcaactctatctgcactgctcattaatatacttctgg |
| 08/23, 08/24 | Deletion for Upstream Forward | gagcaaatccccttattgggggtaagacatg |
| 08/23, 08/24 | Deletion for Upstream Reverse | ctctagaagcggccgcgaattcc |
| 09/13, 09/22 | Add J23105 Forward | CAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc |
| 09/13, 09/22 | Add J23105 Reverse | AGCTAGCCGTAAActctagtatataaacgcagaaaggcccacc |
| - | VF2 (<partinfo>G00100</partinfo>) | tgccacctgacgtctaagaa |
| - | VF (<partinfo>G00101</partinfo>) | attaccgcctttgagtgagc |
Reagent
| Name | Supplier |
|---|---|
| Ampicillin | |
| Kanamycin | |
| Chloramphenicol | |
| EcoRI | |
| XbaI | |
| SpeI | |
| PstI | |
| DpnI | |
| Isopropyl β-D-1-Thiogalactopyranoside (IPTG) | |
| Ligation High |
