Team:HokkaidoU Japan/Notebook/August27
From 2010.igem.org
(Difference between revisions)
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=Transfer 1-1A on pSB1A10 to pSB1C3= | =Transfer 1-1A on pSB1A10 to pSB1C3= | ||
1.estimated concentration of pSB1C3 and 1-1A which was amplified by miniprep before | 1.estimated concentration of pSB1C3 and 1-1A which was amplified by miniprep before | ||
- | |||
+ | 2.mixed solutions according to the table below | ||
+ | <div style="float:left;"> | ||
{|style="text-align: center;" class="protocol" | {|style="text-align: center;" class="protocol" | ||
|- | |- | ||
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|style="border-top:1px solid #996"|'''100 uL''' | |style="border-top:1px solid #996"|'''100 uL''' | ||
|} | |} | ||
+ | </div> | ||
+ | <div style="float:left; margin-left:50px;"> | ||
{|style="text-align: center;" class="protocol" | {|style="text-align: center;" class="protocol" | ||
|- | |- | ||
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|style="border-top:1px solid #996"|'''20 uL''' | |style="border-top:1px solid #996"|'''20 uL''' | ||
|} | |} | ||
+ | </div> |
Revision as of 12:03, 13 October 2010
ラオリ英語添削お願い
Transfer 1-1A on pSB1A10 to pSB1C3
1.estimated concentration of pSB1C3 and 1-1A which was amplified by miniprep before
2.mixed solutions according to the table below
Reagent | Amount |
---|---|
pSB1C3 | 5 uL |
DW | 75 uL |
10x M Buffer | 10 uL |
1%BSA | 10 uL |
EcoRI | 5 uL |
PstI | 5 uL |
Total | 100 uL |
Reagent | Amount |
---|---|
1-1A | 8 uL |
DW | 6 uL |
10x M Buffer | 2 uL |
1%BSA | 2 uL |
EcoRI | 1 uL |
PstI | 1 uL |
Total | 20 uL |