Team:HokkaidoU Japan/Notebook/August20

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{{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/August19|August 19]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/August23|August 23]]</div></div>
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=PCRによるVectorの増幅=
=PCRによるVectorの増幅=
[[Image:HokkaidoU Japan 20100820a.JPG‎|200px|right|thumb|]]
[[Image:HokkaidoU Japan 20100820a.JPG‎|200px|right|thumb|]]

Revision as of 14:54, 18 September 2010

Notebook

PCRによるVectorの増幅

HokkaidoU Japan 20100820a.JPG

pSB1C3, pSB1T3, pSB1A3について，それぞれ以下の組成でPCRを行った

DW 　34 uL
10x buffer 　5 uL
2mM dNTP 　5 uL
25mM MgSO4 　3 uL
Suffix-F 　0.5 uL
Prefix-R 　0.5 uL
KOD 　1 uL
DNA Template 　1 uL
Total 　50 uL
  • pSB1T3が増えていなかった
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