Team:HokkaidoU Japan/Notebook/August20

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=PCRによるVectorの増幅=
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=Amplified vector by [[Team:HokkaidoU_Japan/Protocols|PCR]]=
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[[Image:HokkaidoU Japan 20100820a.JPG‎|200px|right|thumb|]]
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pSB1C3, pSB1T3, pSB1A3について，それぞれ以下の組成でPCRを行った
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[[Image:HokkaidoU Japan 20100820a.JPG‎|200px|right|thumb|Electrophoresis to confirm amplification]]
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{|style="margin-left:30px;"
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Amplified pSB1C3, pSB1T3, pSB1A3 accordingly to the table bellow.
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{| class="protocol"
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|'''Reagent'''
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|'''Amount'''
|-
|-
|DW
|DW
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|　1 uL
|　1 uL
|-
|-
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|style="border-top:1px solid #000;"|'''Total'''
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|style="border-top:1px solid #996;"|'''Total'''
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|style="border-top:1px solid #000;"|　'''50 uL'''
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|style="border-top:1px solid #996;"|　'''50 uL'''
|}
|}
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* pSB1T3が増えていなかった
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* pSB1T3 failed to amplify

Latest revision as of 08:37, 2 October 2010

Notebook

Amplified vector by PCR

Electrophoresis to confirm amplification

Amplified pSB1C3, pSB1T3, pSB1A3 accordingly to the table bellow.

Reagent Amount
DW 　34 uL
10x buffer 　5 uL
2mM dNTP 　5 uL
25mM MgSO4 　3 uL
Suffix-F 　0.5 uL
Prefix-R 　0.5 uL
KOD 　1 uL
DNA Template 　1 uL
Total 　50 uL
  • pSB1T3 failed to amplify
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