Team:HokkaidoU Japan/Notebook/August20
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- | {{Template:HokkaidoU_Japan}} | + | {{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/August19|August 19]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/August23|August 23]]</div></div> |
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+ | =Amplified vector by [[Team:HokkaidoU_Japan/Protocols|PCR]]= | ||
+ | |||
+ | [[Image:HokkaidoU Japan 20100820a.JPG|200px|right|thumb|Electrophoresis to confirm amplification]] | ||
+ | |||
+ | Amplified pSB1C3, pSB1T3, pSB1A3 accordingly to the table bellow. | ||
+ | |||
+ | {| class="protocol" | ||
+ | |'''Reagent''' | ||
+ | |'''Amount''' | ||
+ | |- | ||
+ | |DW | ||
+ | | 34 uL | ||
+ | |- | ||
+ | |10x buffer | ||
+ | | 5 uL | ||
+ | |- | ||
+ | |2mM dNTP | ||
+ | | 5 uL | ||
+ | |- | ||
+ | |25mM MgSO<sub>4</sub> | ||
+ | | 3 uL | ||
+ | |- | ||
+ | |Suffix-F | ||
+ | | 0.5 uL | ||
+ | |- | ||
+ | |Prefix-R | ||
+ | | 0.5 uL | ||
+ | |- | ||
+ | |KOD | ||
+ | | 1 uL | ||
+ | |- | ||
+ | |DNA Template | ||
+ | | 1 uL | ||
+ | |- | ||
+ | |style="border-top:1px solid #996;"|'''Total''' | ||
+ | |style="border-top:1px solid #996;"| '''50 uL''' | ||
+ | |} | ||
+ | |||
+ | * pSB1T3 failed to amplify |
Latest revision as of 08:37, 2 October 2010
Amplified vector by PCR
Amplified pSB1C3, pSB1T3, pSB1A3 accordingly to the table bellow.
Reagent | Amount |
DW | 34 uL |
10x buffer | 5 uL |
2mM dNTP | 5 uL |
25mM MgSO4 | 3 uL |
Suffix-F | 0.5 uL |
Prefix-R | 0.5 uL |
KOD | 1 uL |
DNA Template | 1 uL |
Total | 50 uL |
- pSB1T3 failed to amplify