Team:Harvard/color/parts

From 2010.igem.org

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<h2>RNAi constructs</h2>
<h2>RNAi constructs</h2>
<table cellspacing="0">
<table cellspacing="0">
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<tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Resistance (Plants)</b></td><td><b>Sequence</b></td></tr>
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<tr><td><b>Biobrick Name</b></td><td><b>Description</b></td><td><b>Size</b></td><td><b>Sequence</b></td></tr>
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<tr><td>V1</td><td>pORE Open Series 1 (O1)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>pat</td><td></td></tr>
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<tr><td>BBa_K382010</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown</td><td>413</td><td><a href="https://2010.igem.org/Team:Harvard/c1">link</a></td></tr>
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<tr><td>V2</td><td>pORE Open Series 2 (O2)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>BBa_K382011</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>413</td><td><a href="https://2010.igem.org/Team:Harvard/c2">link</a></td></tr>
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<tr><td>V3</td><td>pORE Expression Series 3 (E3)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>pat</td><td></td></tr>
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<tr><td>BBa_K382012</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>413</td><td><a href="https://2010.igem.org/Team:Harvard/c3">link</a></td></tr>
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<tr><td>V4</td><td>pORE Expression Series 4 (E4)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V5</td><td>pORE Reporter Series 1 (R1)</td><td>Agrobacterium vector with gusA reporter</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V6</td><td>pORE Reporter Series 3 (R3)</td><td>Agrobacterium vector with smgfp reporter</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V7</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td></td></tr>
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<tr><td>V8</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V9</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td></td></tr>
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<tr><td>V10</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V11</td><td><center>-</center></td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V12</td><td><center>-</center></td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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</table>
</table>
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<h2>Primers</h2>
<h2>Primers</h2>
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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>
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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>
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<tr><td>VP3</td><td>HindIII-pENTCUP2 forward primer for Expression Series insert</td><td></td></tr>
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<tr><td>CP1</td><td>LUT2 I</td><td>gaTAACTTCCTAGTAACCCGCATtctctcttttgtattcc</td></tr>
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<tr><td>VP4</td><td>pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert</td><td></td></tr>
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<tr><td>CP2</td><td>LUT2 II</td><td>gaATGCGGGTTACTAGGAAGTTAtcaaagagaatcaatga</td></tr>
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<tr><td>VP5</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>CP3</td><td>LUT2 III</td><td>gaATACGGGTTACTACGAAGTTTtcacaggtcgtgatatg</td></tr>
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<tr><td>VP6</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>CP4</td><td>LUT2 IV</td><td>gaAAACTTCGTAGTAACCCGTATtctacatatatattcct</td></tr>
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<tr><td>VP7</td><td>HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>CP13</td><td>BETA-OHASE I</td><td>gaTCGTTTAAATCGAAGAGACGCtctctcttttgtattcc</td></tr>
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<tr><td>VP8</td><td>smGFP-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>CP14</td><td>BETA-OHASE II</td><td>gaGCGTCTCTTCGATTTAAACGAtcaaagagaatcaatga</td></tr>
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<tr><td>VP9</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr>
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<tr><td>CP15</td><td>BETA-OHASE III</td><td>gaGCATCTCTTCGATATAAACGTtcacaggtcgtgatatg</td></tr>
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<tr><td>VP11</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr>
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<tr><td>CP16</td><td>BETA-OHASE IV</td><td>gaACGTTTATATCGAAGAGATGCtctacatatatattcct</td></tr>
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<tr><td>VP12</td><td>1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>CP9</td><td>LYC I</td><td>gaTAATTATCCAACGACTTGCAGtctctcttttgtattcc</td></tr>
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<tr><td>VP13</td><td>2 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>CP10</td><td>LYC II</td><td>gaCTGCAAGTCGTTGGATAATTAtcaaagagaatcaatga</td></tr>
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<tr><td>VP14</td><td>3 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>CP11</td><td>LYC III</td><td>gaCTACAAGTCGTTGCATAATTTtcacaggtcgtgatatg</td></tr>
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<tr><td>VP15</td><td>4 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>CP12</td><td>LYC IV</td><td>gaAAATTATGCAACGACTTGTAGtctacatatatattcct</td></tr>
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<tr><td>VP16</td><td>5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>VP17</td><td>1 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP18</td><td>2 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP19</td><td>3 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP20</td><td>4 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP21</td><td>5 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP22</td><td>6 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP23</td><td>7 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP24</td><td>8 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP25</td><td>9 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP26</td><td>1 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP27</td><td>2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP28</td><td>3 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP29</td><td>4 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP30</td><td>5 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP33</td><td>1 of 2 for sequencing V7 and V8 biobrick cloning site</td><td></td></tr>
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<tr><td>VP34</td><td>2 of 2 for sequencing V7 and V8 biobrick cloning site</td><td></td></tr>
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</table>
</table>
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Latest revision as of 00:25, 27 October 2010



parts and primers

RNAi constructs

Biobrick NameDescriptionSizeSequence
BBa_K382010amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown413link
BBa_K382011amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown413link
BBa_K382012amiRNA construct for lycopene beta cyclase (LYC) knockdown413link


Primers

NameDescriptionSequence
CP1LUT2 IgaTAACTTCCTAGTAACCCGCATtctctcttttgtattcc
CP2LUT2 IIgaATGCGGGTTACTAGGAAGTTAtcaaagagaatcaatga
CP3LUT2 IIIgaATACGGGTTACTACGAAGTTTtcacaggtcgtgatatg
CP4LUT2 IVgaAAACTTCGTAGTAACCCGTATtctacatatatattcct
CP13BETA-OHASE IgaTCGTTTAAATCGAAGAGACGCtctctcttttgtattcc
CP14BETA-OHASE IIgaGCGTCTCTTCGATTTAAACGAtcaaagagaatcaatga
CP15BETA-OHASE IIIgaGCATCTCTTCGATATAAACGTtcacaggtcgtgatatg
CP16BETA-OHASE IVgaACGTTTATATCGAAGAGATGCtctacatatatattcct
CP9LYC IgaTAATTATCCAACGACTTGCAGtctctcttttgtattcc
CP10LYC IIgaCTGCAAGTCGTTGGATAATTAtcaaagagaatcaatga
CP11LYC IIIgaCTACAAGTCGTTGCATAATTTtcacaggtcgtgatatg
CP12LYC IVgaAAATTATGCAACGACTTGTAGtctacatatatattcct

Retrieved from "http://2010.igem.org/Team:Harvard/color/parts"