Team:Harvard/color/parts

From 2010.igem.org



parts and primers

RNAi constructs

Biobrick NameDescriptionSizeSequence
BBa_K382010amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown413link
BBa_K382011amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown413link
BBa_K382012amiRNA construct for lycopene beta cyclase (LYC) knockdown413link


Primers

NameDescriptionSequence
CP1LUT2 IgaTAACTTCCTAGTAACCCGCATtctctcttttgtattcc
CP2LUT2 IIgaATGCGGGTTACTAGGAAGTTAtcaaagagaatcaatga
CP3LUT2 IIIgaATACGGGTTACTACGAAGTTTtcacaggtcgtgatatg
CP4LUT2 IVgaAAACTTCGTAGTAACCCGTATtctacatatatattcct
CP13BETA-OHASE IgaTCGTTTAAATCGAAGAGACGCtctctcttttgtattcc
CP14BETA-OHASE IIgaGCGTCTCTTCGATTTAAACGAtcaaagagaatcaatga
CP15BETA-OHASE IIIgaGCATCTCTTCGATATAAACGTtcacaggtcgtgatatg
CP16BETA-OHASE IVgaACGTTTATATCGAAGAGATGCtctacatatatattcct
CP9LYC IgaTAATTATCCAACGACTTGCAGtctctcttttgtattcc
CP10LYC IIgaCTGCAAGTCGTTGGATAATTAtcaaagagaatcaatga
CP11LYC IIIgaCTACAAGTCGTTGCATAATTTtcacaggtcgtgatatg
CP12LYC IVgaAAATTATGCAACGACTTGTAGtctacatatatattcct

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