Team:Edinburgh/Bacterial
From 2010.igem.org
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Revision as of 10:50, 18 October 2010
Bacterial BRIDGEs
Communication via light.
Our Project
Figure 3: Normalised absorbance spectra of:
a. the lov2 domain of Avena sativa with bound FMN (adapted from Schüttrigkeit et al., 2003).
b. Green light absorbing form of cyanobacteriochrome CcaS from Synechocystis sp. PCC 6803. Adapted from Hirose et al, 2008.
c. Red light absorbing form of phytochrome Cph1-PCB adduct, from Synechocystis sp. PCC 6803. Adapted from Gambetta and Lagarias (2001).
Note that the relative absorbance of each spectrum is normalised to one.
The response of light receptors Cph8, lovTAP and our green light receptor to different wavelengths of light have not been measured. The absorbance spectra of the light sensitive proteins might not exactly mirror their response to light, but should give us a good idea until they are characterized.
In lovTAP, the light sensitive domain of the protein is the lov2 domain from the Avena sativa blue light receptor phototropin. This binds flavin mononucleotide (FMN) which is its co factor. Schüttrigkeit et al. (2003) measured the absorbance of the wild type lov2 bound to FMN (see Figure 3). Since this is the active part of lovTAP, we expect our blue light receptor to have a similar response in vivo. The red light absorbing form of Cph1 is what responds to red light in Cph8. The absorbance of Cph1 was measured by Gambetta and lagarias (2001). Similarly, the absorbance of the green light absorbing form of CcaS, which we are planning on using in our green light receptor, was measured by Hirose et al. (2008).
Table of Contents
- The core repressilator upon which we chose to base our project.
- A description of the work we did on the red light producing component.
- A description of the work we did on the red light sensing component.
- A description of the work we did on the blue light producing component.
- A description of the work we did on the blue light sensing component.
- A description of the work we did on the green light producing component.
- A description of the work we did on the green light sensing component.
- The BioBricks we submitted as part of developing the light producing and light sensing components.
- A summary of what we achieved as part of developing the light producing and light sensing components.
- Our vision of the future of E. coli communication through light, and where we would like to go next.
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References used throughout the section.