Team:DTU-Denmark/Wet lab

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===== Anti-Terminator experimental setup =====
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= Anti-Terminator experimental setup =
=== N-protein plasmid ===
=== N-protein plasmid ===

Revision as of 11:47, 7 September 2010

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Home Team Project Parts Submitted to the Registry Modeling Wet Lab Gallery</span

Anti-Terminator experimental setup

N-protein plasmid

The N protein were isolated from salmonella genomic DNA with specific designed primers. We used the natural occurring RBS site, as a High expression of N have shown non specific anti-termination effect on a global scale on the genome. #References

References

  • NC Franklin, JH Doelling - Am Soc Microbiol "Overexpression of N antitermination proteins of bacteriophages lambda, 21, and P22: loss of N protein specificity." - Journal of bacteriology, 1989