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Team:Cambridge/References/ProjectBioluminescence/Experiments
From 2010.igem.org
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==2. Test use of CHBT as a substrate== | ==2. Test use of CHBT as a substrate== | ||
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With sucessfully transformed cells from 1 compare emission when adding: | With sucessfully transformed cells from 1 compare emission when adding: | ||
| - | + | * D-luciferin | |
| - | + | * CHBT | |
| - | + | * CHBT + D-Cysteine | |
| - | + | * CHBT + L-Cysteine | |
| - | + | * (CHBT + L-Luciferin? if necessary) | |
| - | + | ||
| - | + | ||
| - | + | ||
Revision as of 16:08, 26 July 2010
Firefly
1. Test luminescence with the Luciferase from the registry
- Transform WT E.coli with the luciferase from the registry.
- Feed with D-luciferin, develop a method for measuring light output
- Variables - concentration of inducer (if luciferase promoter inducible), concentration of D-luciferin
- Controls - no luciferin, no inducer
2. Test use of CHBT as a substrate
With sucessfully transformed cells from 1 compare emission when adding:
- D-luciferin
- CHBT
- CHBT + D-Cysteine
- CHBT + L-Cysteine
- (CHBT + L-Luciferin? if necessary)
