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Team:Cambridge/References/ProjectBioluminescence/Experiments
From 2010.igem.org
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*'''a) D-cysteine''' | *'''a) D-cysteine''' | ||
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| - | b) L-cysteine via L-luciferin | + | |
| + | '''b) L-cysteine via L-luciferin''' | ||
Revision as of 16:01, 26 July 2010
Firefly
1. Test luminescence with the Luciferase from the registry
- Transform WT E.coli with the luciferase from the registry.
- Feed with D-luciferin, develop a method for measuring light output
- Variables - concentration of inducer (if luciferase promoter inducible), concentration of D-luciferin
- Controls - no luciferin, no inducer
2. Test use of CHBT as a substrate
- a) D-cysteine
- Luciferase + D-luciferin
b) L-cysteine via L-luciferin
