From 2010.igem.org
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| | ==Wednesday 6/23== | | ==Wednesday 6/23== |
| | + | * Transformation plates are still covered in a film of bacteria. The negative control for the kanamycin test grew bacteria, suggesting that there is an issue with the kanamycin stock. |
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| | ==Thursday 6/24== | | ==Thursday 6/24== |
Revision as of 18:51, 25 June 2010
iGEM 2010
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Monday 6/21
- Transformed additional bricks from distribution with other antibiotic resistances onto plates & liquid culture.
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_I15008 I15008], [http://partsregistry.org/wiki/index.php?title=Part:BBa_I15009 I15009], [http://partsregistry.org/wiki/index.php?title=Part:BBa_I15010 I15010], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0033 E0033], [http://partsregistry.org/wiki/index.php?title=Part:BBa_M30109 M30109], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112000 K112000], [http://partsregistry.org/wiki/index.php?title=Part:BBa_J09250 J09250]
Tuesday 6/22
- Transformation success is ambiguous - the plates are covered in a lawn of bacteria, apparently indicating an issue with the antibiotic selection. This implies either a problem with the stock solution of antibiotic (Kan/Strep), or the procedure of plating the antibiotics on the surface of the LB-agar plates.
- To find out what happened, we set up a simple experiment:
- Reperformed the transformations on two of the bricks & plated on LB-agar plates with the antibiotic incorporated.
Wednesday 6/23
- Transformation plates are still covered in a film of bacteria. The negative control for the kanamycin test grew bacteria, suggesting that there is an issue with the kanamycin stock.
Thursday 6/24
Friday 6/25
Weekend 6/26-27
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