Team:Caltech/Week 2

From 2010.igem.org

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==Tuesday 6/22==
==Tuesday 6/22==
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* Transformation success is ambiguous - the plates are covered in a lawn of bacteria, apparently indicating an issue with the antibiotic selection. This implies either a problem with the stock solution of antibiotic (Kan/Strep), or the procedure of plating the antibiotics on the surface of the LB-agar plates.
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** To find out what happened, we set up a simple experiment:
 +
* Reperformed the transformations on two of the bricks & plated on LB-agar plates with the antibiotic incorporated.
==Wednesday 6/23==
==Wednesday 6/23==

Revision as of 22:58, 22 June 2010


iGEM 2010



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Contents

Monday 6/21

  • Transformed additional bricks from distribution with other antibiotic resistances onto plates & liquid culture.
    • [http://partsregistry.org/wiki/index.php?title=Part:BBa_I15008 I15008], [http://partsregistry.org/wiki/index.php?title=Part:BBa_I15009 I15009], [http://partsregistry.org/wiki/index.php?title=Part:BBa_I15010 I15010], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0033 E0033], [http://partsregistry.org/wiki/index.php?title=Part:BBa_M30109 M30109], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112000 K112000], [http://partsregistry.org/wiki/index.php?title=Part:BBa_J09250 J09250]

Tuesday 6/22

  • Transformation success is ambiguous - the plates are covered in a lawn of bacteria, apparently indicating an issue with the antibiotic selection. This implies either a problem with the stock solution of antibiotic (Kan/Strep), or the procedure of plating the antibiotics on the surface of the LB-agar plates.
    • To find out what happened, we set up a simple experiment:
  • Reperformed the transformations on two of the bricks & plated on LB-agar plates with the antibiotic incorporated.

Wednesday 6/23

Thursday 6/24

Friday 6/25

Weekend 6/26-27

 
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