Team:Caltech/Week 1

From 2010.igem.org

(Difference between revisions)
(Thursday)
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*Made stock solutions for transformation and subsequent culturing
*Made stock solutions for transformation and subsequent culturing
*Transformed XL-1 Blue E. Coli with selected BioBricks
*Transformed XL-1 Blue E. Coli with selected BioBricks
 +
**J09250, K124017, K124014, R0082, R0015, R0034, pBAD, I13601
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 +
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==Friday==
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*Checked transformation
 +
*Minipreped transformed bacteria
 +
**Checked the plasmid concentration in the mini-prep solution
 +
*Made glycerol stocks from the transformed plasmid

Revision as of 23:40, 18 June 2010

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Contents

Monday

  • Discussed procuring items & lab space.
  • Prepared for SURF start.

Tuesday

  • Created GANTT Chart (below) detailing our work plan for the summer.
GANTT Chart diagramming our planned progress throughout the summer.
  • Detailed possible side projects, open to any member as time permits:
    • Agarase
    • pH/Environmental Response
    • Phototaxis
    • Colored plastic
    • Activa (transglutaminase)
  • Procured supplies from stock room
  • Cleaned out lab space
  • Prepared LB-amp media & plates

Wednesday

  • Procured remaining antibiotics & sample of XL1 Blue cells
  • Prepare orders for Sigma, NEB, and others
    • Sigma: Crosslinking compounds, substrates (linseed oil & soybean oil)
    • NEB: Biobrick assembly kit, Ladders, polymerase
  • Prepared list of biobricks to order
    • Addl Lysis bricks
    • Agarase bricks

Thursday

  • Made stock solutions for transformation and subsequent culturing
  • Transformed XL-1 Blue E. Coli with selected BioBricks
    • J09250, K124017, K124014, R0082, R0015, R0034, pBAD, I13601


Friday

  • Checked transformation
  • Minipreped transformed bacteria
    • Checked the plasmid concentration in the mini-prep solution
  • Made glycerol stocks from the transformed plasmid