Team:Caltech/BBa K338003

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Note that these three genes should only cause the production of PHB ''oligomers'' in cells, not hardened plastic. A crosslinking agent is required to link the oligomers and form the final plastic product. Over-expression of the ''phaC1'' gene could cause some crosslinking, but this has not been experimentally verified.
Note that these three genes should only cause the production of PHB ''oligomers'' in cells, not hardened plastic. A crosslinking agent is required to link the oligomers and form the final plastic product. Over-expression of the ''phaC1'' gene could cause some crosslinking, but this has not been experimentally verified.
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====BioBrick Characterization====
 
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=====Effect of Heat Shock Temperature=====
 
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Revision as of 10:28, 26 October 2010


iGEM 2010



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<partinfo>BBa_K338003 short</partinfo>

This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: phaA, phaB1, phaC1. This half contains only phaA: <partinfo>BBa_K156012</partinfo>. It was designed to be ligated upstream of part BBa_K338004.

Usage and Biology

Design

When ligated upstream of BBa_K338004, the completed construct was designed to express all three PHA synthase genes required to make PHB oligomers from soybean oil. The three genes would be transcribed polycistronically on a single mRNA transcript under the IPTG-inducible control of the <partinfo>BBa_K215000</partinfo> promoter. Naturally, each gene is preceded by a standard RBS (<partinfo>B0034</partinfo>) and the transcript finishes with a strong terminator (<partinfo>B0015</partinfo>), for a total size of about 3500bp.

Note that these three genes should only cause the production of PHB oligomers in cells, not hardened plastic. A crosslinking agent is required to link the oligomers and form the final plastic product. Over-expression of the phaC1 gene could cause some crosslinking, but this has not been experimentally verified.


 
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