Team:Calgary/20 July 2010


Revision as of 21:08, 20 July 2010 by Raidakhwaja (Talk | contribs)

Tuesday July 20, 2010

Gel electrophoresis of Raida's PCR products: BBK primers that anneal before the multiple cloning sites. Lane 1 and 2: Lux0047 A + B0015 psB1AK3 Lane 3: J23002 + Lux0047E, Lane4: R0040 + I3502, Lane 5: Lux0047E, Lane 6: Master Mix


Today I set up and ran two 1% gels: one for my PCR products from yesterday and the second one for Emily's PCR products- both of which were done to test whether our primers are working or not. Please see the gel to the side: As we can see in the gel electrophoresis image, the expected results has been obtained. For example, Lane 5 contains only the Lux0047E gene where as Lane 3 has J23002 + Lux0047E, hence the band in Lane 5 goes slightly further relative to Lane 3 because it is a shorter band. Lanes 1 and 2 show exactly the same band because they are the same genes in the same plasmid backbone. Furthermore, Lane 6 shows no band because it is the Master Mix and our negative control. Therefore, it can be concluded that the primers are functional.

No notebook page exists for this date. Sorry!