Team:Alberta/PartsDesigner
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- | ==== | + | ====Open Reading Frames==== |
- | [[Image:Alberta- | + | [[Image:Alberta-Gfp.png|frame|left|A GFP open reading frame]] |
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+ | Open reading frames are blocks of various colors with a short acronym or abbreviation of its name on them. The ends are given a 'torn away' appearance that interlocks with the symbols for start and stop codons. This is to remind the user that BioByte open reading frame parts do not contain a start or stop codon. Therefore, the user must remember to proceed an ORF BioByte with a start codon, and include a stop codon after. Otherwise, interesting gene fusions could be created, intentionally or not. | ||
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- | [[Image:Alberta- | + | ====Selectable Markers==== |
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+ | [[Image:Alberta-Ampr.png|frame|left|An ampicillin resistance open reading frame]] | ||
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Revision as of 01:13, 26 October 2010
The Plasmid Designer
The plasmid designer gives students and researchers the ability to design and validate their plasmids in in silico. Through an intuitive drag and drop interface, users can piece their plasmids together BioByte by BioByte. During this, the program provides feedback. It can check whether the desired plasmid will circularize properly. The presence of a selectable marker and an origin of replication is also checked. The user can see the resulting annotated sequence of the plasmid as it is being built.
Part Graphics
In order to make the interface as intuitive as possible, icon graphics were chosen that would provide clear visual feedback of the key structures and functional sequences present on the plasmid.
The A and B Ends
'A' sticky ends are denoted as two interlocking red pieces, and 'B' sticky ends are denoted as two differently interlocking blue pieces. This gives a quick reminder that 'AB' parts can only be stuck to 'BA' parts in sequence, and vice versa.
Origins of Replication
Origins of replication are given a unique block shape with the symbol, 'ORI' written on them.
Open Reading Frames
Open reading frames are blocks of various colors with a short acronym or abbreviation of its name on them. The ends are given a 'torn away' appearance that interlocks with the symbols for start and stop codons. This is to remind the user that BioByte open reading frame parts do not contain a start or stop codon. Therefore, the user must remember to proceed an ORF BioByte with a start codon, and include a stop codon after. Otherwise, interesting gene fusions could be created, intentionally or not.
Selectable Markers
Promoters
Ribosome Binding Sites
Start and Stop Codons
Transcriptional Terminators