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Team:Alberta/PartsDesigner
From 2010.igem.org
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Revision as of 01:03, 26 October 2010
The Plasmid Designer
The plasmid designer gives students and researchers the ability to design and validate their plasmids in in silico. Through an intuitive drag and drop interface, users can piece their plasmids together BioByte by BioByte. During this, the program provides feedback. It can check whether the desired plasmid will circularize properly. The presence of a selectable marker and an origin of replication is also checked. The user can see the resulting annotated sequence of the plasmid as it is being built.
Part Graphics
In order to make the interface as intuitive as possible, icon graphics were chosen that would provide clear visual feedback of the key structures and functional sequences present on the plasmid.
The A and B Ends
An 'AB' Part
A 'BA' Part
Origins of Replication
An origin of replication
Selectable Markers
An ampicillin resistance open reading frame
Open Reading Frames
A GFP open reading frame
Promoters
A promoter followed by a ribosome binding site and a start codon
Ribosome Binding Sites
Start and Stop Codons
A stop codon followed by a ribosome binding site and a start codon
Transcriptional Terminators
A stop codon followed by a transcriptional terminator
