Korea U Seoul/2 October 2010
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(New page: ===Ligation of each parts : PyodA-mAAA-PzntA-RFP, ParsR-GFP and pSB1C3=== <BR> {| style="border: 1px solid red;" |Reagent |Volume (μL) |- |10x T4 DNA ligase reaction buffer |2 |- |T4 DN...)
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(New page: ===Ligation of each parts : PyodA-mAAA-PzntA-RFP, ParsR-GFP and pSB1C3=== <BR> {| style="border: 1px solid red;" |Reagent |Volume (μL) |- |10x T4 DNA ligase reaction buffer |2 |- |T4 DN...)
Newer edit →
Revision as of 13:45, 20 October 2010
Ligation of each parts : PyodA-mAAA-PzntA-RFP, ParsR-GFP and pSB1C3
Reagent | Volume (μL) |
10x T4 DNA ligase reaction buffer | 2 |
T4 DNA ligase | 2 |
Each of the digests | 2 + 2 + 2 = 8 |
D.W. | 8 / total = 20 |
Incubation at room temperature for 30min
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