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Korea U Seoul/2 October 2010
From 2010.igem.org
Ligation of each parts : PyodA-mAAA-PzntA-RFP, ParsR-GFP and pSB1C3
| Reagent | Volume (μL) |
| 10x T4 DNA ligase reaction buffer | 2 |
| T4 DNA ligase | 2 |
| Each of the digests | 2 + 2 + 2 = 8 |
| D.W. | 8 / total = 20 |
| Incubation at room temperature for 30min and stop at 80˚C for 20min | |
|---|---|
