Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test17agosto

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AUGUST, 17TH


PLATE


UNIPV Pavia piastra17agosto.png


EXPERIMENT DESCRIPTION

Motivation

The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.

Methods

The experiment was performed in LB according to Protocol 1 on the following measurement parts:

  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
  • <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)

RESULTS

Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:

UNIPV Pavia FluoVSODHC1708.png
UNIPV Pavia FluoVSODCo1708.png

The ODstart are reported in the table below:

Part ODstart
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> 0.1456
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> 0
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> 0.3257
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> -
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> 0.5068
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> 0.1609
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> 0.3098
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> 0.1463
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> -



Next Test

Tecan Test

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