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AUGUST, 18TH
PLATE
EXPERIMENT DESCRIPTION
Motivation
The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.
Methods
The experiment was performed in LB according to Protocol 1 on the following measurement parts:
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
- <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name I15-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
RESULTS
Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:
The ODstart are reported in the table below:
| Part | ODstart
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| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> | 0.1655
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| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> | -
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| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> | -
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| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> | 0.5421
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| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> | 0.4837
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> | 0.1503
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> | 0.3587
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> | -
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> | 0.1729
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> | -
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> | -
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